FAMILY BACILLACEAE 



733 



calidolaciis is the same organism as 

 Bacillus lactis termophilus (sic) Gorini 

 (Giorn. d. R. Soe. Ital. d'Igiene, 16, 

 1894, 16). From the descriptions this 

 appears to be probable. 



Spores: Ellipsoidal, of slightly greater 

 diameter than the rods, terminal. 



Sporangia: Slightly swollen, clavate. 



Rods : 0.7 to 1.4 by 2.6 to 5.0 microns, 

 occurring singly, in pairs and short 

 chains. Non-motile. Gram-positive, 

 some cells becoming Gram-negative with 

 age. 



Gelatin stab: Xo liquefaction. 



No growth on plain nutrient agar. 



Glucose agar colonies: Thin, white, 

 opaque, filamentous. 



Glucose agar slant : Growth abundant, 

 echinate, dull, white. 



Glucose agar stab: Growth abundant, 

 beaded, gray. 



Glucose broth: Turbid. 



Litmus milk: Acid, coagulation. Lit- 

 mus reduced. 



Potato: No growth. 



Nitrites produced from nitrates by 

 some strains. 



Acid from glucose, lactose, fructose, 

 galactose and maltose. No acid from 

 inulin, sucrose or glycerol. 



Thermophilic, optimum temperature 

 55=C to 65°C. No growth at 37°C. 



Aerobic, facultative. 



Source: Isolated from normal pasteur- 

 ized skim milk (Hussong and Hammer). 

 Milk and milk powder (Prickett, N. Y. 

 Agr. Exp. Sta. Tech. Bui. 147, 1928, 47). 



Habitat : Probably in dairy products. 



27. Bacillus michaelisii Prickett. 

 {Bacillus thennophilus aquatilis lique- 

 faciens Michaelis, Arch. f. Hyg., 36, 

 1899, 285; Prickett, N. Y. Agr. Exp. 

 Sta. Tech. Bui. 147, 1928, 45.) Named 

 for Georg Michaelis of Berlin who first 

 isolated the species. 



Spores : Of greater diameter than the 

 rods, terminal. 



Sporangia: Swollen, clavate. 



Rods: 0.6 to 0.8 by 2 to 4 microns. 

 Motile. Gram -positive. 



Gelatin stab : Liquefaction. 



Agar colonies : Circular, raised, smooth, 

 glistening. 



Agar slant : Growth moderate, smooth, 

 glistening. 



Broth: Slight turbidity. 



Milk: Not coagulated, alkaline. 



Potato : Growth moist, glistening, yel- 

 lowish, becoming brownish. 



Nitrites with gas produced from 

 nitrates. 



Starch is hydrolyzed. 



Acid from glucose and sucrose. No 

 acid from rhamnose, maltose, lactose, 

 glycerol, mannitol or inulin. 



Thermophilic, optimum temperature 

 50°C to 60°C. 



Aerobic, facultative. 



Source : Isolated from fountain waters 

 (Michaelis). From fodder, dust, dairy 

 utensils (Prickett). 



Habitat : Probably found in soil and 

 dust. 



27a. Bacillus lohatus Bergey et al. 

 (Type 3, Bergey, Jour. Bact., I^, 1919, 304 ; 

 Bergey et al., Manual, 1st ed., 1923, 311.) 

 From Greek lohatos, having the form of a 

 lobe. 



Judging from the meager description, 

 there is no essential difference between 

 this organism and the preceding. 



Source: Isolated from dust, soil, and 

 horse manure. 



Habitat : Probably widely distributed 

 in soil and decaying matter. 



Bacillus nondiastalicus Bergey et al. 

 (Type 2, Bergey, Jour. Bact., 4, 1919, 304 ; 

 Bergey et al.. Manual, 1st ed., 1923, 310.) 

 From Greek, no diastase. 



The description of this organism is 

 practically identical with Bacillus loba- 

 tus, the only difference noted being that 

 this species hydrolyzes starch while 

 Bacillus nondiastalicus does not. 



Source : Isolated from dust and soil 

 (Bergey). Ground grains, raw and pas- 

 teurized milk (Prickett, N. Y. Agr. E.xp. 

 Sta. Tech. Bui. 147, 1928, 47). 



Thermobacillus vulgaris Feirer (Soil 

 Sci., ^3, 1927, 50) liquefies gelatin, does 



