734 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



not reduce nitrates to nitrites nor alter 

 litmus milk. According to Feirer it is 

 otherwise similar to Bacillus nondiasta- 

 ticus. 

 Source : Two strains isolated from soil . 



27b. Bacillus tlierinunouliquefaciens 

 Bergey et al. (Type 4, Bergey, Jour. 

 Bact., 4, 1919, 304; Bergey et al., Manual, 

 1st ed., 1923, 312.) From Greek thermos, 

 hot; and Latin non, not and liquefaciens, 

 making liquid. Probably intended to 

 mean thermophilic and non-gelatin- 

 liquefying. 



Aside from the non-liquefaction of 

 gelatin, there seems to be no difference in 

 the description of this organism and the 

 two immediately preceding. 



Source: Isolated from dust, soil, and 

 horse manure. 



Habitat: Probably found in soil and 

 decaying matter. 



28. Bacillus thermotranslucens Bergey 

 et al. (Type 5, var. b, Bergey, Jour. 

 Bact., 4, 1919, 304; Bergey et al.. Manual, 

 1st ed., 1923, 312.) From Greek thervws, 

 hot and Latin irausluceits, translucent. 

 Probably intended to mean thermophilic 

 and translucent. 



Spores: Of larger diameter than the 

 rods, terminal. 



Sporangia : Terminally swollen, clavate, 

 not in chains. 



Rods : 0.3 to 0.4 by 1.0 to 1.5 microns, 

 occurring singly. Motile with peritrich- 

 ous flagella. Gram-positive. 



Gelatin stab: No liquefaction. 



Agar colonies: Thin, transparent, 

 spreading widely. 



Agar slant: Growth thin, spreading, 

 veil-like. 



Broth: Turbid. 



Litmus milk: Not coagulated, slightly 

 acid. 



Potato: No growth. 



Nitrites not produced from nitrates. 



Starch slightly hydrolyzed. 



Thermophilic, optimum temperature 

 60°C. Slight growth at 37°C. No growth 

 at 70°C. 



Aerobic . 



Source: Isolated from guinea pig feces, 

 dust and from cheese. 



Habitat : Probably found in soil and 

 decaying matter. 



Thermohacillus linearius Feirer (Soil 

 Sci., 23, 1927, 53) is said to be similar in 

 some respects to the preceding. Feirer 

 states that formation of acid from several 

 sugars is the distinctive feature of this 

 species, a character not mentioned by 

 Bergey. 



Source: Five strains isolated from soil. 



2Sa. Bacillus slearothermophilus Donk. 

 (Jour. Bact., 5, 1920, 373.) From Greek 

 sledr, tallow and thermofhilos, heat- 

 loving. Intended meaning obscure. 



From the descriptions, the vegetative 

 rods of this organism seem to be slightly 

 larger than the preceding, otherwise no 

 difference is noted. 



Source : Isolated from samples of 

 spoiled canned corn and string beans. 



Habitat: Probably found in soil and 

 dust. 



28b. Bacillus aerothermophilus Wein- 

 zirl. (Jour. Med. Research, 39, 1919, 

 403.) From Greek aeros, air and /';e?'- 

 mophihis, heat-loving. Probably in- 

 tended to mean aerobic and thermophilic. 



There is nothing in the original account 

 of this organism which is at variance 

 with that of the preceding. 



Source : Isolated from canned string 

 beans (Weinzirl). From milk, water, 

 hay, dust, beef extract, and agar (Prick- 

 ett, N. Y. Agr. Exp. Station Tech. Bull. 

 147, 1928,46). 



Habitat : Probably found in soil and 

 dust . 



Thermobacilhis alcalinus Feirer (Soil 

 Sci., 23, 1927, 52) is said to differ from 

 the preceding in that it does not change 

 litmus milk. 



Source : Four strains isolated from soil. 



Thermobacillus ruber Feirer (Soil Sci., 

 23, 1927, 52) apparently is closelj' related 

 to this group. Its distinguishing char- 

 acter is the production of a pink pigment 



