FAMILY BACILLACEAE 



735 



in meat, brain, and blood serum, no color 

 on other media. 



Source : Isolated from soil. 



29. Bacillus thermocellulolyticus Cool- 

 haas. (Cent. f. Bakt., II Abt., 76, 1928, 

 43.) From Greek thermos, hot; and 

 Latin cellula, a small room; M. L., cellu- 

 lose and Greek lytikos, dissolving. Prob- 

 ably intended to mean thermophilic and 

 cellulose-digesting. 



Spores: Ellipsoidal, 0.8 by 1.5 microns, 

 terminal. 



Sporangia : Terminally swollen, clavate. 



Rods : 0.3 by 3.5 to 6 microns, occurring 

 singly and in pairs . Xo reserve material . 

 Non-motile. Gram-positive. 



Gelatin stab: Xo liquefaction. 



Glucose agar colonies : Small, glisten- 

 ing, translucent. 



Cellulose agar colonies : Circular, bor- 

 ders undulate to lobate. 



Broth : Slight growth, no surface growth 

 or sediment. 



Milk: No growth. 



Nitrites not produced from nitrates. 



Starch is hydrolyzed. 



No acid from carbohydrates. 



Cellulose hydrolyzed. 



Thermophilic, optimum temperature 

 50°C to 55°C. Maximum 60°C to 65°C. 

 ^Minimum 35°C to 37°C. 



Aerobic, facultative. 



Source: Isolated from sewage. 



Habitat : Probably found in decaying 

 matter. 



30. Bacillus thermoalimentophilus 



Weinzirl. (Jour. Med. Research, 39, 

 1919, 404.) From Greek thermos, hot; 

 Latin alimentum, food; and Greek 

 ■philos, loving. Loving hot food. 



Spores: Ellipsoidal, 0.8 by 1.0 micron, 

 terminal. 



Sporangia: Swollen, clavate, not in 

 chains. 



Rods: 0.6 by 3.0 microns, occurring 

 singly, with rounded ends. Motile, 

 flagella not stated. Gram-positive. 



Gelatin stab : No growth at 20°C. 



Agar colonies : Circular, raised, smooth, 

 amorphous, entire. 



Agar slant : Growth spreading to effuse, 

 smooth, glistening, butyrous. 



Broth: Turbid, surface ring. 



Litmus milk: Unchanged. 



Potato : Xo growth. 



Nitrites with gas produced from ni- 

 trates. 



Starch not hydrolyzed. 



Neither acid nor gas from glucose, lac- 

 tose, sucrose or raannitol. 



Thermophilic, optimum temperature 

 55°C. Xo growth at 20°C. Growth at 

 37°C. 



Aerobic. 



Source : Isolated from canned blueber- 

 ries (Weinzirl). Pasteurized milk and 

 filter cloth (Prickett, X. Y. Agr. Exp. 

 Sta. Tech. Bui. 147, 1928, 46). 



Habitat : Probably found in soil and 

 dust. 



ThermubaciUus violaceus Feirer (Soil 

 Sci., 33, 1927, 52) corresponds in some 

 respects with the preceding. Feirer also 

 states that his cultures did not reduce 

 nitrates to nitrites and produced acid on 

 glucose and sucrose. 



Source : Four strains isolated from soil. 



31. Bacillus thermoliquefaciens Ber- 

 gey et al. (Type 5, var. a, Bergey, Jour. 

 Bact., 4, 1919, 304 ; Bergey et al., Manual, 

 1st ed., 1923, 313.) From Greek thermos, 

 hot, and Latin liquefaciens, liquefying. 

 Probably intended to mean thermophilic 

 and gelatin-liquefying. 



Spores: Ellipsoidal, polar, of greater 

 diameter than the rods. 



Sporangia : Terminally swollen, clavate. 



Rods: 0.2 to 0.4 by 2 to 3 microns, 

 occurring singly, with rounded ends. 

 Motile with peritrichous flagella. Gram- 

 positive. 



Gelatin stab : Liquefaction. 



Agar colonies : ^Moderately dense, 

 lobate. 



Agar slant : Growth dense, grayish, 

 lobate to fimbriate margins. 



Litmus milk: Coagulated, acid. Lit- 

 mus reduced. 



