736 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Potato : No growth. 



Nitrites and ammonia produced from 

 nitrates. 



Starch not hydrolyzed. 



Thermophilic, optimum temperature 

 60°C. Slight growth at 37°C. No growth 

 at 70°C. 



Aerobic . 



Source: Isolated from dust, guinea pig 

 feces and horse manure (Bergey ) . Water 

 and milk (Prickett, N. Y. Agr. Exp. Sta. 

 Tech. Bui. 147, 1928, 47). 



Habitat : Probably originally from soil 

 ■ nd dust. 



32. Bacillus tostus Blau. (Cent. f. 

 Bakt., II Abt., 15, 1905, 130.) From 

 Latin tostus, parched, dried. 



Spores: Ellipsoidal, 0.8 to 1.6 by 1.5 

 to 2.2 microns. Germination prevail- 

 ingly polar. 



Sporangia : Terminally swollen, clavate, 

 not in chains. 



Rods : 1.2 by 4.5 to 5.0 microns, occur- 

 ring in pairs and in short chains. Cells 

 store glycogen. Motile with peritrichous 

 flagella. 



Agar colonies: Small, circular, dense. 

 By transmitted light bright yellow to 

 yellowish-brown. Borders sharp, entire 

 to lobate. Older colonies porcelain-like. 



Agar slant : Growth thin, grayish-white, 

 spreading, smooth, glistening. 



Potato : No growth. 



Nitrites not produced from nitrates. 



Starch is hydrolyzed. 



Ammonia is produced. 



Thermophilic, optimum temperature 

 60°C to 70°C. 



Aerobic. 



Source: Two cultures isolated from 

 soil in Germany. 



Habitat: Probably found in soil and 

 dust. 



33. Bacillus viridulus (Migula) Bergey 

 et al. {Bacillus thermophihis II Rabino- 

 witsch, Ztschr. f. Hyg., 20, 1895, 154; 

 Bacterium viridulum Migula, Syst. der 

 Bakt.,;g, 1900, 343; Bacterium thermophi- 

 lum 7/ Chester, Manual, 1901,186; BaciZ- 

 lus thermophilus Bergey et al.. Manual, 



1st ed., 1923, 315; Bergey et al.. Manual, 

 4th ed., 1934, 464; not Bacillus thermo- 

 philus MiqueL Ann. d. Microg., 1, 1888, 

 6; not Bacillus thermophilus Chester, 

 Man. Determ. Bact., 1901, 265.) From 

 Latin, dim. adj. viridis, green, some- 

 what green. 



Spores: Si)herical, central. 



Sporangia: Cylindrical, not swollen. 



Rods : Rather large, slightly bent, 

 occurring singly and in pairs. Non- 

 motile. Gram-positive. 



Agar colonies : Irregular, spreading, 

 granular, greenish. 



Broth: Alkaline. 



Potato : Growth grayish-yellow ; margin 

 undulate. 



Nitrites not produced from nitrates. 



Starch is hydrolyzed. 



Thermophilic, grows at 60°C to 70°C. 

 Optimum temperature 62°C. Grows at 

 33°C. 



Aerobic, facultative. 



Source : Isolated from soil, snow, feces, 

 corn grains. 



Habitat : Probably found in soil and 

 dust. 



Appendix : The following additional 

 aerobic spore-forming bacteria are found 

 in the literature. Because of insufficient 

 data it has not been possible to classify 

 them. Some of these may be synonyms 

 of well-known species, some may be 

 varieties, whereas others may actually be 

 separate species. 



Aromabacillus weigmanni Omeliansky. 

 (Isolated by Weigmann, 1890; Omelian- 

 sky, Jour. Bact., 8, 1923, 398.) From 

 milk. 



Bacillus abysseus ZoBell and Upham. 

 (Bull. Scripps Inst, of Oceanography, 

 Univ. Calif., 5, 1944, 273.) Subterminal 

 spores. From marine mud. 



Bacillus acidifaciens Patrick and 

 Werkman. (Iowa State Coll. Jour. Sci., 

 7, 1933, 413.) One of a group charac- 

 terized by the fermentation of xylan. 

 A single culture isolated from decayed 

 maple wood. 



Bacillus acidificans presamigenes casei 



