766 MANUAL OF DETERMINATIVE BACTERIOLOGY 



d. Motile. 



e. Gelatin, or glucose gelatin, liquefied, 

 f. Glucose fermented, 

 g. Coagulated albumin liquefied. Milk slowly coagulated. 

 Clot slowly digested. 



20. Clostridium bifermentans. 

 gg. Coagulated albumin not recorded. 



h. Milk slowly coagulated; slimy, 

 i. Gas formed from glucose. 



21. Clostridium mucosum. 

 ii. Acid but no gas from glucose. 



22. Clostridium pruchii. 

 ee. Iron-gelatin (Spray), no growth. 



23. Clostridium cylindrosporum. 

 dd. Non-motile. 



e. Gelatin, or glucose gelatin, liquefied, 

 f. Glucose fermented, 

 g. Coagulated albumin not liquefied. 



h. Milk stormily fermented. Clot not digested, 

 i. Glycerol fermentation variable, 

 j. Mannitol not fermented. Starch, lactose and su- 

 crose fermented. Salicin rarely fermented. 

 Types identified by specific toxin-antitoxin 

 neutralization. 



24. Clostridium perfringens 



Types A, B, C and D. 

 bb. Spores spherical. 



c. Rods distinctly swollen at sporulation. 

 d. Motile. 



e. Gelatin, or glucose gelatin, not liquefied, 

 f. Glucose fermented, 

 g. Coagulated albumin not liquefied. 



h. Milk acidified; slowly and softly coagulated; not 

 stormily. Clot not digested. 



25. Clostridium sphenoides. 

 hh. Milk acidified but not coagulated. 



26. Clostridium innominatum. 

 cc. Rods not swollen at sporulation. 

 d. Non-motile. 



e. Gelatin, or glucose gelatin, not liquefied, 

 f. Glucose fermented, 

 g. Coagulated albumin not liquefied, 

 h. Milk acidified but not coagulated. 

 27. Clostridium filiforme. 

 aa. Spores terminal. 



b. Spores distinctly oval to ellipsoid, 

 c. Rods distinctly swollen at sporulation. 

 d. Motile. 



e. (5elatin, or glucose gelatin, not liquefied. Also see eee. 

 f. Glucose fermented. 



