r86 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



liorder. A little gas is occasionally 

 formed. 



Broth: Transient uniform turbidity, 

 quickly settling as a heavy, white, 

 fiocculent deposit. Culture assumes a 

 cheesy odor. 



Milk: Abundant growth, with lab- 

 coagulation in 2 to 3 days. No increase in 

 acidity; becoming slightly alkaline. Clot 

 completely digested in 10 to 14 days, leav- 

 ing a white, semi-translucent fluid of 

 cheesy odor. 



Indole not formed. 



Ammonia not formed. 



Hydrogen sulfide not formed. 



Glucose not fermented. 



Carbohydrates not fermented. 



Egg medium : Xo digestion or other 

 visible change. 



Coagulated albumin not digested or 

 blackened. 



Blood serum not digested or blackened. 



Meat medium not digested or black- 

 ened, even in presence of metallic iron. 

 Meat particles slightly reddened. 



Brain medium not digested or 

 blackened. 



Grows well between 22°(; and 37°C. 



Anaerobic. 



Non-pathogenic to guinea pigs on sub- 

 cutaneous inoculation (Cunningham, 

 Cent. f. Bakt., II Abt., 83, 1931, 12). 



Source : Originally isolated by Cunning- 

 ham as a dissociant from a culture of 

 Bacillus saccharobutyricus von Klecki. 

 Later isolated by MacLennan; 1 strain 

 from a culture of Clostridium, sporogenes , 

 and 2 strains from street dust. 



Habitat: Not determined, other than 

 these sources. 



18. Clostridium subterminale (Hall 

 and Whitehead) comb. nov. {Bacillus 

 subterminalis Hall and Whitehead, Jour. 

 Inf. Dis., 4i, 1927, 66.) Named from the 

 characteristic position of the spores. . 



Rods : Occurring singly, in pairs and 

 rarely in short chains. Motile. Spores 

 oval, subterminal, swelling rods. Gram- 

 positive. 



Gelatin: Slowly liquefied, with slight 

 turbidity and black sediment. 



Blood agar surface colonies (anaerobic) : 

 Delicate. At first mildly, later actively, 

 hemolytic. 



Deep agar colonies: Opaque, compact, 

 biconvex or lobate discs. 



Agar slant (anaerobic): No surface 

 growt h . 



Glucose broth : Turbidity, but no acid 

 or gas formed. 



Indole not formed. 



Milk: Slowly coagulated (2 to 3 days), 

 with mild acidity and gas. Slow but com- 

 plete digestion of casein (8 to 18 days). 



Glucose, fructose, galactose, maltose 

 and lactose not fermented. 



Brain medium : Slight turbidity in 

 supernatant fluid. Slight gas formation 

 and slow digestion. 



Iron brain medium : Blackened in 2 to 3 

 days . 



Tyrosiu crystals not observable. 



Non-pathogenic to guinea pigs on sub- 

 cutaneous injection. 



Grows well at 37°C. 



Obligately anaerobic. 



Source: From an African arrowhead. 



Habitat : Not determined, other than 

 this source. 



19. Clostridium malenominatum 



(Weinberg et al.) comb. nov. (Pseudo-coli 

 anaerobic, Jungano, Compt. rend. Soc. 

 Biol., Paris, 65, 1908-09, 457; Bacillus 

 pseudo-coli anaerobic Jungano and Dis- 

 taso, Les Anadrobies, 1910, 162; Bacillus 

 pseudocoli a7iacrobius LeBlaye and Gug- 

 genheim, Man. Prat. d. Diag. Bact., 1914, 

 345; Bacillus malenominatus Weinberg 

 et al . , Les Microbes Ana^robies, 1937, 763 ; 

 Paraplectrum malenominatum Prevot, 

 Ann. Inst. Past., 61, 1938, 75.) From 

 Latin, meaning badly named. 



Rods: Short, cocco-bacillary, becom- 

 ing elongated to short filaments in old 

 cultures — especially in sugar broth. 

 Ends rounded. Distinct bipolar staining 

 tendency. Non-motile. Capsulated, 

 especially in body fluids. Spores oval, 



