■88 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Agax' surface colonies (anaerobic ) : 

 Circular, crenated to amoeboid. 



Blood agar surface colonies (anaerobic) : 

 Small, transparent, hemolytic, becoming 

 opaque, yellowish, spreading. 



Broth : Turbidity and gas. Thick mu- 

 coid sediment. 



Litmus milk : Slowly coagulated. 

 Slowly peptonized, with little gas. 



Indole is formed. 



Nitrites not produced from nitrates. 



Hydrogen sulfide is produced. 



Acid and gas from glucose, fructose, 

 mannose and maltose. Lactose, sucrose 

 and inulin not fermented. Records sug- 

 gest variability in glycerol and salicin. 



Coagulated albumin rapidly liquefied 

 and blackened. 



Blood serum liquefied and blackened. 



Brain medium digested and blackened. 



Egg -meat medium digested and black- 

 ened. Tyrosin crystals in 8 to 10 days. 



Pathogenicity: Variable with the 

 strain; some kill rabbits in 24 hours; 

 others produce only slight edema, while 

 some show no effect. 



Toxicity : Likewise variable, from acute 

 to none. 



Optimum temperature from 30°C to 

 37°C. Can grow at 50°C. 



Anaerobic. 



Source: Originally from putrid meat; 

 subsequently from gaseous gangrene. 



Habitat: Occurs commonly in feces, 

 soil and sewage. Widely distributed in 

 nature. 



21. Clostridium mucosum (Klein) Ber- 

 geyetal. {Bacillus 7)mcosus Klein, Cent, 

 f. Bakt., I Abt., 29, 1901, 991; not Ba- 

 cillus 7nucosus Zimmermann, Die Bakt. 

 unserer Trink- u. Nutzwasser, Chem- 

 nitz, 2, 1894, 8; Bacterium mucosum 

 Migula, Syst. d. Bakt., 2, 1900, 315; 

 Bacillus kleinii Buchanan and Hammer, 

 Iowa Agric. Exp. Sta. Res. Bull. 22, 1915, 

 276; not Bacillus kleinii Aligula, Syst. d. 

 Bakt., 2, 1900, 766; not Bacillus kleinii 

 Trevisan, in Hit. cited from De Toni and 

 Trevisan, in Saccardo, Sylloge Fun- 

 gorum, 8, 1889, 946; Clostridium kleinii 



Bergey et al., Manual, 1st ed., 1923, 321; 

 Bergey et al., Manual, 4th ed., 1934, 

 472; not Clostridium mucosum Simola, 

 cited from Prevot, Man. d. Class., etc., 

 1940, 112; Endosporus mucosus Prevot, 

 Ann. Inst. Past., 61, 1938, 75.) From 

 Latin, slimy. 



Rods : 1.3 by 2.0 to 5.0 microns, occur- 

 ring singly, in pairs and in chains. Mo- 

 tile. Spores oval, central, not swelling 

 rods. Gram-negative (Klein, loc. cit.). 

 Young cultures Gram-positive (Buchanan 

 and Hammer, loc. cit.). 



No growth in media without carbohy- 

 drates. 



Glucose gelatin: Slowly liquefied. 



Glucose gelatin surface colonies (anae- 

 robic) : Small, gray. 



Glucose gelatin stab : Villous growth. 

 Slow liquefaction. 



Glucose agar slant (anaerobic): Thin, 

 veil-like layer. Slimy condensation 

 water. 



Glucose broth: Turbid. Gas bubbles. 



Litmus milk: Acid; slowly coagulated, 

 slimy. Gas formed. Odor of butyric 

 acid. 



Potato : No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Acid and gas from glucose. 



Blood serum : No growth. 



Non-pathogenic. 



Grows at 37°C. 



Anaerobic. 



Source: Blood sausage (Blutwurst). 



Habitat : Not determined, other than 

 this source. 



22. Clostriditim pruchii (Buchanan and 

 Hammer) Bergey et al. {Bacillus lactis 

 pruchii Conn, Esten and Stocking, 18th 

 Ann. Rept. Storrs Agric. Exp. Sta., 1906, 

 179; Bacillus pruchii Buchanan and 

 Hammer, Iowa Agric. Exp. Sta. Res. Bull. 

 No. 22, 1915, 276; Bergey et al.. Manual, 

 1st ed., 1923, 322.) Named for M. J. 

 Prucha, American bacteriologist. 



Rods: Variable in size, with club- 

 shaped ends. Motile, with peritrichous 



