792 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



maturation, swelling rods. Gram-posi- 

 tive only in young cultures. 



Gelatin : Not liquefied. 



Agar surface colonies (anaerobic ) : 

 Circular, or slightly irregular, entire. 



Blood agar surface colonies (anaerobic) : 

 Minute dew-drops, becoming whitish, 

 opaque. Blood is hemolyzed. 



Deep agar colonies : Minute, opaque, 

 smooth disks. 



Broth: Turbid. 



Litmus milk: Acid; slowly and softly 

 coagulated. Clot not digested. 



Indole not formed (indole formed by 

 Tholby strain, Stanley and Spray, Jour. 

 Bact., 4^, 1941, 256). 



Nitrites produced from nitrates. 



Acid and gas from glucose, galactose, 

 maltose, lactose and salicin. Inulin, 

 glycerol and dulcitol not fermented. 

 Strains are apparently variable on manni- 

 tol, sucrose, dextrin and starch. 



Coagulated albumin not liquefied. 



Blood serum not liquefied. 



Brain medium not blackened or di- 

 gested. 



Non-pathogenic for guinea pig and 

 rabbit. 



Optimum temperature not determined. 

 Grows well at 30°C to 37°C. 



Anaerobic . 



Source: From gangrenous war wounds. 



Habitat : Not determined, other than 

 this source. 



26. Clostridium innominatum Prevot. 

 (Bacillus E, Adamson, Jour. Path, and 

 Bact., 22, 1918-19, 391; Pr(5vot, Ann. 

 Inst. Past., 6i, 1938,85.) From Latin, 

 remaining unnamed. 



Rods: Very small, thick, tapering at 

 one or both ends, occurring singly, paired, 

 in chains and filaments. Involution 

 forms abundant on glucose agar. Motile. 

 Spores small, spherical, subterminal, 

 swelling rods. Gram-positive, quickly 

 becoming Gram-negative. 



Gelatin : Not liquefied. 



Glucose agar surface colonies (anaero- 

 bic) : Two forms are produced : 1) Circu- 



lar, entire edge, opaque; 2) Diffuse, 

 spreading, irregular and translucent. 



Plain agar surface colonies (anaerobic) : 

 Small, circular, entire edge, whitish- 

 translucent, becoming opaque-yellowish 

 with age. 



Plain broth : Moderate turbidity, clear- 

 ing by sedimentation in 3 to 4 days. 



Glucose broth : More abundant tur- 

 bidity and slight gas production. 



Milk: Slowly acidified but not clotted. 

 No further change. 



Glucose, maltose, lactose and mannitol 

 fermented with acid and gas. 



Sucrose not fermented. 



Coagulated albumin : Not digested or 

 blackened. 



Meat medium : Not digested or black- 

 ened. 



Blood serum : Not digested or black- 

 ened. 



Brain medium : Not digested or black- 

 ened. 



Non -pathogenic (Prevot, loc. cil.). 



Grows well at 37°C. 



Anaerobic. 



Source : From septic and gangrenous 

 war wounds. 



Habitat : Not determined, other than 

 this source. 



27. Clostridiiun filiforme Bergey et al. 



(Bacillus regularis filiformis Debono, 

 Cent. f. Bakt., I Abt., Grig., 62, 1912, 

 234; Bergey et al., Manual, 1st ed., 1923, 

 331.) From Latin, thread-like. 



Rods : 0.5 to 0.8 by 3.0 to 5.0 microns, 

 slender, occurring singly, in pairs, in 

 chains and filaments. Non-motile. 

 Spores very small, spherical, subterminal, 

 or occasionally terminal, not swelling 

 rods. Gram -positive. 



Gelatin : Not liquefied. 



Deep gelatin colonies: Small, gray, 

 filamentous. 



Deep agar colonies : Irregular, gray, 

 translucent, filamentous. 



Broth: Uniform turbidity. 



Litmus milk: Acid, but no further 

 change. 



