FAMILY BACILLACEAE 



797 



Habitat : Not determined, other than 

 these sources. 



36. Clostridiiun zoogleicum Bergey et 

 al. {Bacillus sporogenes zoogleiciis Dis- 

 taso, Cent. f. Bakt., I Abt., Orig., 59, 

 1911, 99; Bergey et al.. Manual, 1st ed., 

 1923, 335.) From Greek, zoogleal. 



Rods : Fairly long, occurring singly, in 

 pairs and in short chains. Motile. 

 Spores large, oval, terminal, swelling 

 rods. Gram-positive. 



Gelatin : Growth and liquefaction not 

 recorded. 



Deep agar colonies: Small, gray, 

 slightly opaque, becoming heart-shaped. 

 Gas is not formed. 



Broth: Turbid, then clearing with 

 zoogleal sediment. 



Litmus milk: Slowly coagulated, then 

 digested. Litmus reduced. 



Indole is formed in trace. 



Acid but no gas from glucose. Lactose 

 and sucrose not fermented. 



Coagulated albumin liquefied, leaving a 

 clear fluid and zoogleal sediment . 



Grows at 37°C. 



Anaerobic. 



Source : From human feces. 



Habitat : L'ndetermined, other than 

 this source. 



37. Clostridium thermosaccharolyticum 

 McClung. (Jour. Bact., 29, 1935, 200; 

 Terminosporus thernwsaccharolyticus 



Prevot, Ann. Inst. Past, 61, 1938, 86.) 

 From Greek, heat, and sugar-digesting. 



Rods: 0.4 to 0.7 by 3.5 to 7.5 microns, 

 slender, granulated, occurring singly and 

 in pairs, not in chains. Motile with peri- 

 trichous flagella. Spores spherical, ter- 

 minal, swelling rods. Gram -negative. 



Gelatin : Not liquefied. 



Pea-infusion agar surface colonies 

 (anaerobic): Granular, grayish-white, 

 raised center, with feathery edges. 



Deep glucose-tryptone agar colonies : 

 Small, lenticular, smooth. 



Liver-infusion broth over liver meat : 

 Turbidity and gas. 



Litmus milk: Litmus reduced. Acid 



and slow but firm coagulation; coagulum 

 split with gas. Clot not digested. 



Indole not formed. 



Nitrites not produced from nitrates. 



Cellulose not fermented. 



Acid and gas from arabinose, fructose, 

 galactose, glucose, mannose, xylose, cello- 

 biose, lactose, maltose, sucrose, trehalose, 

 dextrin, glycogen, corn-starch, amygda- 

 lin, esculin, a-methyl glucoside and 

 salicin. Raffinose weakly fermented. 

 Rhamnose, inulin, pectin, erythritol, 

 inositol, mannitol, glycerol, quercitol and 

 Ca-lactate not fermented. 



Coagulated albumin not liquefied. 



Blood serum not liquefied. 



Brain medium not blackened or di- 

 gested. 



Meat-medium not blackened or di- 

 gested. 



Non-pathogenic on feeding to white 

 rat, or by injection into rabbit. 



Optimum temperature 55°C to 62°C. 

 Thermophilic. 



Anaerobic. 



Source : From hard-swell of canned 

 goods, and from soil. 



Habitat: Not determined, other than 

 these sources. 



38. Clostridium caloritolerans Meyer 

 and Lang. (Jour. Inf. Bis., 39, 1926, 321 ; 

 Plectridium caloritolerans Prevot, Ann. 

 Inst. Past., 61, 1938, 87.) From Latin, 

 heat-enduring. 



Rods : 0.5 to 0.8 by 8.0 to 10.0 microns, 

 with rounded ends, occurring singly, in 

 pairs, in chains and in curved filaments. 

 Motile with peritrichous flagella. Spores 

 spherical or pear-shaped, terminal, swell- 

 ing rods. Gram-positive. 



Gelatin : Not liquefied. 



Glucose blood agar surface colonies 

 (anaerobic): Small, flat, grayish, rhizoi- 

 dal. Non-hemolytic. 



Deep liver agar colonies: Small, flat, 

 transparent disks with large polar tufts. 

 Some colonies become flufi^y. 



Broth: Slight turbidity. 



Glucose broth : Abundant turbidity. 



