798 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



with clearing by sedimentation. Gas is 

 formed. 



Brom cresol purple milk : No change. 



Indole not formed. 



Acid and gas from glucose, galactose 

 and maltose. Fructose feebly fer- 

 mented. Lactose, sucrose, raffinose, inu- 

 lin, salicin, mannitol, inositol and glyc- 

 erol not fermented. 



Coagulated albumin not liquefied. 



Blood serum not liquefied. 



Brain medium not blackened or di- 

 gested. 



Beef -heart mash medium : Reddened ; 

 not blackened or digested. 



Non-pathogenic for mouse, guinea pig 

 and rabbit. 



Optimum temperature not determined. 

 Grows at 37°C. 



Anaerobic . 



Source: From an old culture of Clos- 

 tridium parabotulinum Type A. 



Habitat: Not determined, other than 

 this single isolation. 



39. Clostridium tetanoides (Adamson) 

 Hauduroy et al. (Unnamed anaerobe, 

 Adamson and Cutler, Lancet, 1, 1917,688; 

 Bacillus tetanoides (A) Adamson, Jour. 

 Path, and Bact., 22, 1918-19, 382; Hau- 

 duroy et al., Diet. d. Bact. Path., 1937, 

 140; Palmula macrospora Pr^vot, Ann. 

 Inst. Past., 61, 1938, 88; Acuformis ma- 

 crosporus Prevot, Man. d. Class., etc., 

 1940, 166.) From Latin, tetanus-like. 



Rods : 1.0 to 2.0 by 4.0 to 12.0 microns 

 (averaging 1.0 to 1.5 by 6.0 to 7.0 mi- 

 crons), with rounded to slightly tapered 

 ends, occurring singly, in pairs and in 

 chains of 3 to 5 cells, but not in filaments. 

 Motile only in young cultures. Spores 

 large, spherical, terminal, swelling rods. 

 Gram-positive in young cultures, soon 

 becoming Gram-negative. 



Gelatin : Not liquefied. 



Glucose agar surface colonies (anaero- 

 bic) : Circular, regular, opaciue, bluish- 

 gray, moist, shining, thick, raised. 

 Surface flat, Ijecoming conical in center 

 with age. On moist medium showing 



radiating, dendritic branching. Growth 

 becomes tenacious -mucoid. 



Plain agar surface colonies (anaerobic ) : 

 Confluent, becoming an opaque film. 

 Isolated colonies circular to slightly ir- 

 regular. Dendritic branching and mu- 

 coid tendency less evident than on glucose 

 agar. 



Glucose agar stab : Thick growth along 

 stab, starting 0.5 cm below surface. 

 No gas or splitting of medium. 



Neutral-red glucose agar: Reduced to 

 orange by transmitted, and greenish- 

 fluorescent by reflected light. 



Plain broth: Early slight turbidity, 

 with clearing and mucoid sedimentation. 



Glucose broth : Abundant turbidity and 

 profuse mucoid sediment. 



Milk : Slight and slowly increasing alka- 

 linity, with slow separation of casein. 

 No further change. 



Indole : Trace formed in broth. 



Glucose and maltose fermented with 

 acid but no gas. Lactose, sucrose, manni- 

 tol, starch and cellulose not fermented. 



Coagulated albumin : Not digested or 

 blackened. 



Meat medium : Not digested or black- 

 ened. 



Blood serum : Not digested or black- 

 ened. 



Brain medium : Not digested or black- 

 ened. 



Non-pathogenic for guinea pig and 

 rabbit. 



Optimum temperature not recorded. 

 Grows well at 37°C. 



Anaerobic . 



Source: From war wounds, from post- 

 mortem blood culture, and from garden 

 soil. 



Habitat: Not determined, other than 

 these sources. 



40. Clostridium tetani (Fliigge) Hol- 

 land. (Tetanusbacillen and Tetanuser- 

 reger, Nicolaier, Deuts. Med. Wchnschr.. 

 10, 1884, 843; Bacillus tetani Fliigge, Die 

 Mikroorg., 2 Aufl., 1886, 274; Pacinic 

 nicolaieri Trevisan, I generi e le speci* 

 delle Batteriacee, 1889, 23; Plectridium 



