800 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



24, 1932, 61, and, as it cannot be definitely 

 related to any other anaerobic species 

 (including the Eiweissbacillus, Bien- 

 stock, loc. cit., Bacillus putrificus coli 

 Fltigge, loc. cit., Bacillus putrificus Bien- 

 stock, loc. cit., etc.), they propose the 

 name of Clostridium, lentoputrescens for 

 this species. 



Rods : 0.4 to 0.6 by 7.0 to 9.0 microns, 

 with rounded ends, occurring singly, in 

 pairs and in chains. Motile with peri- 

 trichous flagella. Spores spherical, ter- 

 minal, swelling rods. Weakly Gram- 

 positive, becoming Gram -negative. 



Gelatin : Liquefied. 



Agar surface colonies (anaerobic) : 

 Small, circular, flat, edge crenated 

 to filamentous spreading. Develop a 

 ground-glass appearance. 



Deep agar colonies : Fluffy spheres with 

 fibrils radiating from a central nucleus. 



Blood agar is hemolyzed. 



Litmus milk: Slow, soft coagulation 

 or flocculent precipitation. Casein is 

 slowly digested. 



Indole is formed (Hall, Jour. Lif. Dis., 

 30, 1922, 141 ) . Not formed (Hartsell and 

 Rettger, loc. cit., 509). 



Nitrites not produced from nitrates. 



Hydrogen sulfide formed in egg-meat 

 medium. 



Carbohydrates not fermented. Glu- 

 cose slightly attacked without distinct 

 acid (Hartsell and Rettger, loc. cit., 508). 



Coagulated albumin slowlj' liquefied 

 and blackened. 



Blood serum is liquefied. Gas is 

 formed. 



Brain medium slowly blackened and 

 digested. 



Egg-meat medium : Slightly turbid 

 liquid. Meat reddened in 7 to 10 days, 

 then digested with a foul odor. 



Non-pathogenic for white mouse, 

 guinea pig and rabbit. Filtrate non-toxic 

 on injection or feeding. 



Grows well at 37°C. 



Anaerobic. 



Source : From putrefying meat. 



Habitat: Intestinal canal of human. 

 Widely dispersed in soil. 



42. Clostridium filamentosum Bergey 

 et al. {Bacillus putrificus filamentosus 

 Distaso, Cent. f. Bakt., I Abt., Orig., 59, 

 1911, 98; Bergey et al.. Manual, 1st ed., 

 1923, 333; Palviula filamentosa Prevot, 

 Ann. Inst. Past., 61, 1938, 88; Acuformis 

 filamentosus Prevot, Man. d. Class., etc., 

 1940, 165.) From Latin, filamentous. 



Rods : Slender, occurring singlj^, in 

 pairs and in chains. Motile. Spores 

 spherical, or nearly so, terminal, swelling 

 rods. Gram-positive. 



Gelatin : Liquefied. 



Deep glucose agar colonies : Delicate, 

 cotton J' flocculi. Only a trace of gas 

 formed . 



Broth : Turbid. 



Litmus milk : May or may not coagulate 

 and digest slowly (variable). 



Indole formed in scarcely detectable 

 trace. Odor of scatol. 



Glucose is feebly fermented, with little 

 gas. Lactose and sucrose not fermented. 



Coagulated albumin : Rendered trans- 

 parent, then slowly liquefied. 



Grows well at 37°C. 



Anaerobic . 



Source : From human feces. 



Habitat : Not determined, other than 

 this source. 



43. Clostridium tetanomorphum (Bul- 

 loch et al.) Bergey et al. (Bacillus 

 pseudo -tetanus, Type No. IX, — Tetanus- 

 like Bacillus (Pseudotetanus Bacillus), 

 Mcintosh and Fildes, Med. Res. Counc, 

 Spec. Rept. Ser. No. 12, 1917, 11 and 

 32; Bacillus tetanomorphus Bulloch et 

 al., Med. Res. Counc, Spec. Rept. Ser. 

 No. 39, 1919, 41; Macintoshillus tetano- 

 morphus Heller, Jour. Bact., 7, 1922, 5; 

 Bergey et al.. Manual, 1st ed., 1923, 330; 

 Plectridium tetanomorphum Prevot, Ann. 

 Inst. Past., 61, 1938, 87.) From Greek, 

 shaped like the tetanus organism. 



Synonyms or possibly related species : 

 Bacillus pscudotetani Migula, Syst. d. 

 Bakt., 2, 1900, 598 (Tetanusahnlicher Ba- 

 cillus and Pseudotetanusbacillus, Tavel 

 and Lanz, Mitteil. a. klin. Med. Inst. d. 

 Schweiz, 1, 1893, 162; Bacillus taveli 



