802 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



angular, opaque, yellowish. Gas bubbles 

 are formed. 



Broth : Turbid. 



Litmus milk: Acid and coagulated in 

 14 days. 



Indole is formed. 



Acid and gas from glucose, lactose and 

 sucrose. Butyric acid is formed. 



Coagulated albumin not liquefied. 

 Odor of skatol. 



Optimum temperature 37°C. 



Anaerobic. 



Distinctive character : Resembles the 

 Bacille neigeux, Jungano, Compt. rend. 

 Soc. Biol., Paris, 62, 1907, 677, in form, 

 but not in other respects. 



Source : From human feces. 



Habitat: Not determined, other than 

 this source. 



46. Clostridium putrefaciens (Mc- 

 Bryde) Sturges and Drake. {Bacillus 

 'pulrejaciens McBryde, U. S. D. A., Bur. 

 An. Ind., Bull. 132, 1911, 6; Sturges and 

 Drake, Jour. Bact., U, 1927, 175; Pal- 

 mula putrefaciens Pr^vot, Ann. Inst. 

 Past., 61, 1938, 89 ; Acuformis putrefaciens 

 Pr^vot, Man. d. Class., etc., 1940, 165.) 

 From Latin, putrefying. 



Description from McBryde {loc. cit.) 

 and amplified from Sturges and Drake 

 {loc. cit.). 



Rods : 0.5 to 0.7 by 3.0 to 15.0 microns, 

 rounded ends, occurring singly, in pairs, 

 and in chains and filaments. Non-motile. 

 Spores spherical, terminal, swelling rods. 

 Gram-positive. 



Gelatin : Liquefied. 



Agar surface colonies (anaerobic ) : 

 Small, filamentous. 



Agar slant (anaerobic) : Scanty, white, 

 beaded, glistening growth. 



Broth: Moderate turbidity. Heavy, 

 fiocculent sediment. 



Litmus milk: Rennet coagulation, 

 peptonized. Litmus reduced. 



Indole not formed. 



Nitrites not produced from nitrates. 



Slight production of hydrogen sulfide. 



Acid and gas from glucose. Lactose, 



sucrose, maltose and starch not fer- 

 mented. 



Coagulated albumin liquefied. 



Blood serum liquefied. 



Brain medium blackened and slowlj'' 

 digested. 



Minced pork medium : Slight disinte- 

 gration; sour, putrefactive odor. 



Non-pathogenic. 



Optimum temperature 20°C to 25°C. 

 Slow growth at 0°C and no visible growth 

 at 37°C. 



Anaerobic. 



Source : From muscle tissue of hogs at 

 slaughter. 



Habitat: Not determined, other than 

 this source. 



47. Clostridium nigrificans Workman 

 and Weaver. (Iowa State Coll. Jour. 

 Sci., 2, 1927-28, 63; Werkman, Iowa State 

 Coll. Research Bull. 117, 1929, 165.) 

 From Latin niger, black and faciens, 

 making. 



Rods : 0.3 to 0.5 by 3.0 to 6.0 microns, 

 with rounded ends. Motile. Spores 

 oval, subterminal, slightly swelling rods. 

 Gram-positive. 



Gelatin : Not liquefied. 



Deep agar colonies : Show blackening of 

 medium around colonies. Black in- 

 creased by adding 0.1 per cent ferric 

 chloride to medium. 



Milk: Not recorded. 



Indole not formed. 



Nitrites not produced from nitrates. 



Glucose not fermented. 



Carbohydrates not fermented. 



Coagulated albumin not liquefied. 



Blood serum not liquefied. 



Brain medium blackened but not 

 digested. 



Hydrogen sulfide produced from 

 cystine. 



Non-pathogenic to man, guinea pig, 

 mouse, rat and rabbit. 



Optimum temperature 55°C. Ther- 

 mophilic, growing at 65°C to 70°C. 



Anaerobic. 



Distinctive character : Black colonies 

 in agar media. 



