FAMILY BACILLACEAE 



803 



Source : From canned corn showing 

 sulfur stinker spoilage; also occasionally 

 from soil and manure. 



Habitat : Presumably soil, although de- 

 tected with great difficulty. 



48. Clostridium belfantii (Carboneand 

 Venturelli) Spray. (Bacillus belfantii 

 Carbone and Venturelli, Boll. 1st. Siero- 

 ter., Milan, 4, 1925, 59; not Bacillus bel- 

 /an<u Migula, Syst. d. Bakt., 2, 1900, 767; 

 Spray, in Manual, 5th ed., 1939, 759; 

 Endosporus belfantii Pr^vot, Ann. Inst. 

 Past., 61, 1938, 75.) Named for Belfanti, 

 an Italian bacteriologist. 



Rods: 0.4 to 0.6 by 1.5 to 7.0 microns, 

 thick and straight, occurring singly, in 

 pairs and in short chains. Motile. 

 Spores large, oval, central to subterminal, 

 swelling rods. Usually Gram-negative, 

 occasional cells Gram-positive, 



Granulose reactior. negative. 



Gelatin : Not liquefied. 



Plain agar surface colonies (anaerobic) : 

 Large, round, opaque, with filamentous 

 edge. 



Deep agar colonies : Arborescent along 

 the stab. Gas is formed. 



Plain broth : Diffuse turbidity, clearing 

 by precipitation. No pigmentation. Gas 

 is formed. 



Potato mash: Forms a foam becoming 

 violet in 24 to 48 hours, persisting 3 to 6 

 days, but disappearing on exposure to air. 



Potato slant : Grayish pellicle, becom- 

 ing violet in 24 to 48 hours. Gas of alco- 

 holic odor is produced. No acetone. 



Glycerinated potato : Thin, grayish 

 pellicle, not becoming violet. 



Milk: Coagulated in 24 to 48 hours. 

 Clot broken by gas. 



Milk agar: Abundant growth. Gas of 

 butyric odor is liberated. 



Indole is formed. 



Hydrogen sulfide not formed. 



Acid and gas from glucose, fructose, 

 maltose, sucrose, lactose and mannitol. 

 Starch and inulin weakly fermented. 



Coagulated albumin not liquefied. 



Blood serum not liquefied. 



Grows well at 37°C. 



Anaerobic . 



Specificallyagglutinatedonly by homol- 

 ogous antiserum. 



Source : From retting beds and from air. 



Habitat : Not determined, other than 

 these sources. 



Note : Six other strains of similar pig- 

 menting, sporulating anaerobes are de- 

 scribed by the authors. These have the 

 general characters of Clostridium bel- 

 fantii, but differ in certain particulars, 

 such as color of pigment, fermentation 

 and specific agglutination. Present in- 

 formation does not permit accurate 

 systematic differentiation. 



48a. Clostridium maggiorai (Carbone 

 and Venturelli) Spray. (Bacillus mag- 

 giorai Carbone and Venturelli, loc. cit., 

 59; Spray, in Manual, 5th ed., 1939, 759; 

 Endosporus maggiorai Prdvot, loc. cit., 

 76.) Named for Maggiora, an Italian 

 bacteriologist. 



Characters in general those of the 

 group, but does not produce indole. 



Violet pigmentation persisting only 24 

 hours . 



No alcoholic odor from cultures. 



Specificallyagglutinatedonly by homol- 

 ogous antiserum. 



From mud from bed of stream in Italy. 



48b. Clostridium derossii (Carboneand 

 Venturelli) Spray. (Bacillus de rossii 

 Carbone and Venturelli, loc. cit., 59; 

 Spray, in Manual, 5th ed., 1939, 760; 

 Endosporus rossii Prdvot, loc. cit., 76.) 

 Named for G. de Rossi, an Italian 

 bacteriologist. 



Characters in general those of the 

 group. Greenish pigmentation on potato 

 slant, changing to violet or orange. 



Indole is formed. 



Spedificallyagglutinatedonly by homol- 

 ogous antiserum. 



From soil in Italy. 



48c. Clostridium ottolenghii (Carbone 

 and Venturelli) Spray. (Bacillus otto- 

 lenghii Carbone and Venturelli, loc. cit., 

 59; Spray, in Manual, 5th ed., 1939, 760; 



