804 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Endosporus ottolenghii Pr^vot, loc. cit., 

 76.) Named for Ottolenghi, an Italian 

 bacteriologist. 



Characters in general those of the 

 group . Potato slant digested to a grayish- 

 brown mash. Greenish pigment changing 

 to reddish. Gas of disagreeable odor is 

 formed. 



Indole is formed. 



Specificallyagglutinatedonly by homol- 

 ogous antiserum. 



From mud from bed of a stream in Italy. 



48d. Clostridium paglianii (Carboneand 

 Venturelli) Spray. {Bacillus paglianii 

 Carbone and Venturelli, loc. cit., 60; not 

 Bacillus paglianii Trevisan, I generi e le 

 specie delle Batteriacee, 1889, 19; Spray, 

 in Manual, 5th ed., 1939, 760; Endosporus 

 paglianii Prdvot, loc. cit., 76.) Named 

 for Pagliani, an Italian bacteriologist. 



Characters in general those of the 

 group. One or two subterminal spores 

 are said to be formed. 



Greenish pigmentation on potato, 

 browning with age. 



Indole is formed. 



Specifically agglutinated only by 

 homologous antiserum. 



From soil in Italy. 



48e. Clostridium lustigii (Carbone and 

 Venturelli) Spray. {Bacillus lustigii 

 Carbone and Venturelli, loc. cit., 59; not 

 Bacillus lustigii Trevisan, in litt., quoted 

 from DeToni and Trevisan, in Saccardo, 

 Sylloge Fungorum, 8, 1889, 958; not 

 Bacillus lustigi Chester, Man. Determ. 

 Bact., 1901, 304; Spray, in Manual, 5th 

 ed., 1939, 760; Endosporus lustigii Pr6vot, 

 loc. cit., 76.) Named for Lustig, an 

 Italian bacteriologist. 



Characters in general those of the 

 group. Green pigmentation on potato 

 slant. 



Indole is not formed. 



Specificallyagglutinatedonly by homol- 

 ogous antiserum. 



From mud and soil in Italy. 



48f. Clostridium sclavoi (Carbone and 

 Venturelli) comb. nov. {Bacillus sclavoi 



Carbone and Venturelli, loc. cit., 60; 

 Endosporus sclavoei Pr^vot, loc. cit., 76.) 

 Named for Sclavo, an Italian bac- 

 teriologist. 



Characters in general those of the 

 group. Greenish to brown pigmentation 

 on potato slant. 



Indole is not formed. 



Specificallyagglutinatedonly by homol- 

 ogous antiserum. 



From retting flax in Italy. 



49. Clostridium venturelli (deTomasi) 

 Spray. {Bacillus venturelli de Tomasi, 

 Boll. 1st. Sieroter. Milanese, 4, 1925, 

 203; Endosporus venturelli Prdvot, Ann. 

 Inst. Past., 61, 1938, 76; Spray, in 

 Manual, 5th ed., 1939, 769.) Named for 

 Venturelli, an Italian bacteriologist. 



Rods: Pleomorphic, fusiform to 

 straight or slightly curved, with rounded 

 ends. Size variable with medium, 0.5 

 to 0.8 by 2.5 to 8.0, and up to 20.0 microns. 

 Occurring singly, in pairs, in chains, or 

 frequently in parallel groupings. Motile. 

 Capsulated. Spores oval, central to ex- 

 centric, swelling rods. Gram-negative. 



Granulose reaction positive; showing 

 violet granules with iodine. 



Gelatin: No growth; no liquefaction. 



Glucose agar surface colonies (an- 

 aerobic) : Round, becoming rose-colored. 



Plain agar slant (anaerobic): No 

 growth. 



Maltose agar stab : Colonies lenticular, 

 yellowish, turning to rose. Odor of 

 acetone. 



Plain broth : No growth. 



Potato slant (anaerobic) : Becomes 

 mucilaginous. Bubbles of gas of amylic 

 odor. 



Potato mash: Very abundant growth; 

 rose color, with red spots. 



Milk with CaCOs : Coagulated; becom- 

 ing yellow, then pale rose. Amylic odor. 



Acid and gas from glucose, maltose, 

 sucrose, fructose, lactose, inositol, dex- 

 trin and starch. Arabinose, glycerol, 

 mannitol and inulin not fermented (cited 

 from Weinberg et al., Les Mic. Anaer., 

 1937,800). 



