i'AMILY BACILLACEAE 



807 



Plain broth : Xo growth. 



Glucose broth: Abundant, uniform 

 turbiditj', with much gas. Yellow slim\' 

 sediment . 



Litmus milk: Acid and coagulation. 

 Litmus reduced. Clot torn and j^ellowed. 

 No visible digestion. 



Potato : Digested to a yellow slime. 

 Much gas with butylic odor. 



Corn mash : Resembling reaction of 

 Clostridium acetobutylicum, but with 

 flesh to orange pigment. 



Indole not formed. 



Nitrates reduced to ammonia. 



Nitrites reduced to ammonia. 



Acid and gas from arabinose, xylose, 

 glucose, mannose, fructose, galactose, 

 lactose, maltose, sucrose, raffinose, starch, 

 dextrin, inulin, glycogen, pectin and 

 salicin. Mannitol, erythritol, gh'cerol, 

 Ca-lactate and cellulose not fermented. 



Fermentation products include butyl 

 and ethyl alcohol, acetone, organic acids 

 (probably butyric and acetic). Ho and 

 COo. 



Coagulated albumin cubes : Softened 

 and yellowed by slow digestion. 



Blood serum not liquefied. 



Brain medium not blackened or 

 digested. 



Non-pathogenic for guinea pig and 

 rabbit . 



Grows at 37°C. 



Anaerobic. 



Source : From retting flax. 



HabLtat : Not determined. Found in 

 soil in Italy. Argentina and in the United 

 States. 



53. Clostridium carbonei Arnaudi. 

 (Soc. Intern. Microbiol., Boll. Sez. Ital., 

 8, 1936, 251, and Boll. 1st. Sieroter, 

 Milano, 16, 1937, 650; Inflabilis carbonei 

 Prevot, Man. d. Class., etc., 1940, 96.) 

 Named for Carbone, an Italian bac- 

 teriologist. 



Rods: 0.8 to 1.0 by 3.5 to 4.5 microns, 

 with ends slightly tapered. Non-motile. 

 Spores oval, terminal, 0.8 to 1.0 by 1.0 to 

 1.75 microns, swelling rods. Gram- 

 positive. 



Granulose reaction strongly positive 

 with iodine solution. 



Gelatin : No growth. 



Glucose and lactose gelatin : No growth. 



Plain agar surface colonies (anaerobic) : 

 Flat, shining, colorless, with irregular 

 edges . 



Malt agar surface colonies (anaerobic) : 

 Creamy to slightly reddish colonies with 

 irregular edges. 



Roux-potato slant (anaerobic) : Puncti- 

 form, raised, opaque, deep red colonies, 

 becoming almost violet. 



Plain agar stab : Only traces of growth 

 along stab. 



Glucose and maltose agar stab : No 

 growth. 



Plain broth: Very slight, colorless, 

 diffuse turbidity. 



Glucose broth : Very slight turbidity. 



Maltose broth: Intense turbidity, with 

 profuse, reddish-yellow sediment. 



Tarozzi broth : Slight, diffuse turbidity. 



Indole not formed. 



Hydrogen sulfide not formed. 



Milk: Soft coagulation, with slight, 

 fine reddish flocculence. Whey turbid 

 and colorless. Reaction acid. Clot not 

 digested. 



Digest-milk (optimum medium) : Very 

 abundant turbidity, with bright red 

 flocculent sediment, diffusing uniformly 

 on shaking. 



Coagulated egg-yolk broth: Slight tur- 

 bidity; no digestion. 



Coagulated egg-albumin broth : Slight 

 turbidity; no digestion. 



Coagulated serum (Loeffler, anaerobic ) : 

 Poor growth; flat, red surface colonies. 

 No digestion. 



Brain medium: Not recorded. 



Cellulose not attacked. Hemp is not 

 retted. 



Ferments weakly: Glucose, maltose, 

 sucrose, galactose, fructose and raffinose. 

 Slow and partial fermentation of lactose 

 (only in acidified medium). Starch 

 slightly fermented. Fermentation pro- 

 ducts include H2, CO), CH4, butyric acid 

 and traces of ethyl alcohol. 



