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MANUAL OF DETERMINATIVE BACTERIOLOGY 



and the reports in certain important 

 respects are conflicting, especially con- 

 cerning pigmentation and utilization of 

 carbohydrates. Aronson, Mudd and 

 Furth found them to differ antigenically, 

 but too few cultures were used to distin- 

 guish between species and strain speci- 

 ficity. 



Source : From tubercles in carp. 



Habitat : The cause of nodule and tu- 

 mor-like formations in carp {Ciprinus 

 carpio). Infectious for carp, frogs, liz- 

 ards. Not infectious for guinea pigs and 

 pigeons. 



7. Mycobacterium marinum Aronson. 

 (Jour. Inf. Dis., 39, 1926, 315.) From 

 Latin marinus, marine. 



Description from Aronson {loc. cit.). 



In lesions, short, thick, uniformly 

 staining organisms are seen frequently 

 occurring in clumps, while long, thin, 

 beaded or barred rods are scattered more 

 discretely. In cultures the organisms 

 have the same appearance. Non-motile. 

 Acid-fast and acid -alcohol -fast. Gram- 

 positive. 



Agar slant (slightly acid) : In five to 

 seven days, moist, glistening, elevated 

 colonies, becoming lemon-yellow. 



Gelatin: Not liquefied. 



Agar colonies : In 5 to 7 days, smooth, 

 moist, slimy, lemon-yellow, later orange- 

 colored. 



Glycerol agar colonies : In 14 to IS 

 days, grayish-white, moist, elevated with 

 irregular margins. Old growths lemon- 

 yellow and still later orange -colored. 



Dorset's and Petroff's egg media: 

 Similar to growth on glycerol agar but 

 more luxuriant. 



Broth and glycerol broth : Growth is 

 diffuse, no pellicle formed. 



Litmus milk : Acidified and coagulated. 



Indole not formed. 



Nitrites not produced from nitrates. 



Carbohydrates : Utilizes arabinose and 

 fructose, fails to utilize sorbitol and 

 galactose (Gordon, Jour. Bact., 84, 1937, 

 617). 



Aerobic, facultative. 



Optimum temperature 18° to 20°C. 

 Fails to survive 60°C for 1 hour, fails to 

 grow at 47°C (Gordon, Jour. Bact., 34, 

 1937,617). 



Pathogenicity : Experimentally infects 

 salt water fish, goldfish, frogs, mice and 

 pigeons, but not rabbits or guinea pigs. 



Antigenic structure : By agglutination 

 and complement fixation distinguished 

 from Mycobacterium ranae, Mycobacte- 

 rium friedmannii, and probably Myco- 

 bacterium piscium (Mudd, Proc. Soc. 

 Exp. Biol, and Med., 23, 1925, 569 ; Furth, 

 Jour. Immunol., 12, 1926, 286). See 

 Mycobacterium piscium . 



Distinctive characters : See Mycobacte- 

 rium piscium. 



Source : From areas of focal necrosis of 

 the liver of sergeant majors (Abudefduf 

 maurilii), croakers (Micropoyon undula- 

 tus) and sea bass (Centropristes striatus). 



Habitat: Causes spontaneous tubercu- 

 losis in salt water fish. 



8. Mycobacterium ranae (Kuster) 

 Bergey et al. (Kiister, Miinch. med. 

 Wchnschr., 52, 1905, 57; Bergey et al., 

 Manual, 1st ed., 1923, 374.) From Latin 

 rana, frog. 



Description from Kuster {loc. cit.), 

 Bynoe (Thesis, McGill University, Mon- 

 treal, 1931) and Aronson (Jour. Inf. Dis., 

 44,1929,222). 



Slender rods, 0.3 to 0.5 by 2 to 8 mi- 

 crons, smaller in old cultures. Uni- 

 formly acid-fast in cultures 2 weeks old 

 or older. In younger cultures the stain- 

 ing is irregular, many organisms are not 

 acid-fast. Non-motile. Gram-positive. 



Gelatin stab: No liquefaction. 



Agar colonies : Irregular, raised colo- 

 nies, 1 to 3 mm in diameter with moist 

 glistening surface, later becoming 

 coarsely granular. 



Agar slant : Thick, spreading, glisten- 

 ing. In old cultures dry and scaly. 

 Putrid odor. Grayish-white. 



Glycerol agar colonies : Similar to gela- 

 tin colonies but slightly creamy and be- 

 coming dry and wrinkled in old cultures. 



