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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Distinctive characters: Saprophyti 

 acid-fast organism, grows rapidly on 

 most media. Shows yellow pigmentation 

 as soon as growth is visible. Grows 

 well on Long's medium lacking glycerol 

 and fails to produce acid when glycerol 

 is present. Survives 60°C for 1 hour 

 and grows at 47°C. 



Source: Originally isolated from hay 

 and grass. Frequently found in soil, 

 dust and other sources. Out of 215 

 cultures of the genus recovered from 

 soils by Gordon (Jour. Bact., 34, 1937, 

 617) Mycobacterium phlei was isolated 

 on 22 occasions. The same author re- 

 ports 3 cultures of a closely related if 

 not identical organism recovered from 

 bovine lymph glands, 1 recovered from 

 bovine skin and 1 recovered from a hen's 

 spleen. 



Habitat: Widely distributed in soils, 

 dust, hay, etc. 



Appendix I : The following saprophytic 

 species have been placed in this genus. 

 Their relationships are not clear. Some 

 are related to or possibly identical with 

 Mycobacterium lacticola. 



Mycobacterium album Sohngen. 

 (Cent. f. Bakt., II Abt., 37, 1913, 599.) 

 From garden earth. 



Mycobacterium bekkerii Bekker. (An- 

 tonie van Leeuwenhoek, 9, 1943, 81 ; abst. 

 in Cent. f. Bakt., I Abt., Orig., 149, 

 1944, 500.) From urine. 



Mycobacterium berolinense Bergey et al . 

 (Tuberkelahnlichen Bacillen, Rabino- 

 witsch, Ztschr. f. Hyg., 26, 1897, 90; 

 Mycobacterium lacticola /3 perrugosum 

 Lehmann and Neumann, Bakt. Diag., 2 

 Aufl., 3, 1899, 410; Mycobacterium lacti- 

 cola perrugosum Haag, Cent. f. Bakt., 

 II Abt., 71, 1927, 3 ; Bergey et al.. Manual, 

 1st ed., 1923, 377.) From butter. 



Mycobacterium butyri Chester. (Man. 

 Determ. Bact., 1901, 357.) This name 

 includes both the Tuberkelahnlichen 

 Bacillen of Rabinowitsch and the Butter 

 Bacillus of Petri. From butter. 



Mycobacterium butyricum Bergey et al. 

 (Butter Bacillus, Petri, Arb. kaiserl. 



cGesundheitsamte, 14, 1898, 1 ; Bergey et 

 al., Manual, 1st ed., 1923, 377.) From 

 butter. 



Mycobacterium cholesterolicum Tak. 

 (Antonie van Leeuwenhoek, 8, 1942, 

 39.) From garden soil. 



Mycobacterium friburgensis (Korn) 

 Chester. (Bacillus friburgensis Korn, 

 Cent. f. Bakt., I Abt., 25, 1899, 532; 

 Mycobacterium lacticola y friburgensis 

 Lehmann and Neumann, Bakt. Diag., 2 

 Aufl., 2, 1899,411 ; Chester, Man. Determ. 

 Bact., 1901, 359.) From butter. 



Mycobacterium graminis Chester. 

 (Grasbacillus II, Moeller, Cent f. Bakt., 

 I Abt., 25, 1899, 369; Mycobacterium lac- 

 ticola a planum Lehmann and Neumann, 

 Bakt. Diag., 2 Aufl., 2, 1899, 408; Chester, 

 Man. Determ. Bact., 1901, 358; Myco- 

 bacterium lacticola planum Haag, Cent, 

 f. Bakt., II Abt., 71, 1927, 3.) From 

 hay dust. 



Mycobacterium hyalinum Sohngen. 

 (Cent. f. Bakt., IFAbt., 37, 1913, 599.) 

 From garden earth. 



Mycobacterium luteum Sohngen ijioc. 

 cit.). From garden earth. 



Mycobacterium muris Simmons. 

 (Jour. Inf. Dis., 4^, 1927, 13.) From the 

 feces of gray mice. 



Mycobacterium phlei perrugosum Haag. 

 (Cent. f. Bakt., II Abt., 71, 1927, 6.) 

 From soils and manure. 



Mycobacterium phlei planum Haag (loc. 

 cit.). From soils. 



Mycobacterium ranicola /and II Haag 

 {loc. cit.). From frogs. 



Mycobacterium rubrum Sohngen {loc. 

 cit.). From garden earth. 



Mycobacterium smegmalis (Trevisan) 

 Chester. (Smegma bacillus, Alvarez 

 and Tavel, Arch. Phys. norm, et path., 

 6, 1SS5, 303; Bacillus smegmatis Trevisan, 

 I generi e le specie delle Batteriacee, 

 1889, 14; Bacterium smegmatis Migula, 

 Syst. d. Bakt., 2, 1900, 497; Chester, 

 Man. Determ. Bact., 1901, 357.) From 

 smegma. Weber (Arb. kaiserl. Gesund- 

 heitsamte, 19, 1902, 251) finds Mycobac- 

 terium smegmatis acid- but not alcohol- 

 fast in contrast to the mammalian 



