FAMILY ACTINOMYCETACBAE 



899 



Distinctive characters : Differs from 

 Nocardia coeliaca and Nocardia actino- 

 morpha in the filiform growth and absence 

 of liquefaction of gelatin. Long rods and 

 filaments . 



Source : Six strains isolated from soils 

 in Great Britain. 



Habitat: Presumablj' soil. 



6. Nocardia leishmanii Chalmers and 

 Christopherson. (A new acid-fast 

 streptothri.x, Birt and Leishman, Jour. 

 Hyg., ^, 1902, 120; Chalmers and Christo- 

 pherson, Ann. Trop. Med. and Parasit., 

 10, 1916, 255; Discomyces leishmani 

 Brumpt, Precis de Parasitol., Paris, 

 3rd ed., 1922, 984; Actinomyces leishmani 

 Sartory and Bailly, Mycoses pul- 

 monaires, 1923, 253.) Named for Leish- 

 man, one of the original isolators. 



Description from Erikson {loc. cit., 

 p. 27). 



Initial cells frequently swollen, large 

 and irregular, aggregated in short chains 

 and then branching out into regular 

 narrow filaments ; at margin of colony on 

 synthetic glycerol agar may be seen 

 comparatively long thick segments with 

 accompanying fringe of normal hyphae ; 

 later entire colonies asteroid in appear- 

 ance, very fine and close angular branch- 

 ing, with aerial hyphae situated singly; 

 aerial mycelium generally abundant with 

 irregularly cylindrical conidia. Slightly 

 acid-fast. The latter property must 

 have been attenuated during artificial 

 cultivation, for the organism is reported 

 as markedly acid-fast by the original 

 isolators. 



Gelatin : Small pink colonies in depths 

 of stab. No liquefaction. 



Glucose agar: Rounded elevated colo- 

 nies with paler frosting of aerial mycelium ; 

 grow^th becoming piled up, aerial myce- 

 lium sparse. 



Glycerol agar: Small round pink colo- 

 nies, tending to be umbilicated and piled 

 up, stiff w^hite aerial spikes. 



Coon's agar : Small round colorless 



colonies, stiff white aerial spikes; later a 

 pink tinge. 



Potato agar : Minute colorless round 

 colonies, small raised patches of white 

 aerial mycelium. 



Dorset's egg medium: Colorless con- 

 fluent growth studded with little wart- 

 like projections bearing stiff aerial spikes ; 

 growi;h becomes pinkish with a white 

 aerial mycelium; later, growth drab 

 gray, medium discolored. 



Serum agar: Minute round colorless 

 colonies with pinkish tinge in confluent 

 raised patch. 



laspissated serum : Small round pale 

 pink colonies, umbilicated and raised up. 



Broth: Liberal growth, white floccu- 

 lent colonies; later pink surface colonies. 



Synthetic sucrose solution : Colorless 

 flocculent sediment, thin colorless 

 pellicle. 



Milk: Surface growth, white aerial 

 mycelium, solid coagulum; later partly 

 peptonized with pink aerial mycelium. 



Litmus milk: Pink surface growth, 

 aerial mycelium, milky opaque after 40 

 days. 



Carrot plug: Small irregularly round 

 raised colonies, colorless, covered vath 

 stiff aerial spikes ; later buff-colored, con- 

 voluted and ribbed growth with small 

 patches of white aerial mycelium; aerial 

 mycelium pink in two months. 



Source : From fatal case of lung disease 

 and pericarditis in man. 



Habitat : Human infections so far as 

 laiown . 



7. Nocardia caprae (Silberschmidt) 

 comb. nov. (Streptothrix caprae Silber- 

 schmidt, Ann. Inst. Past., 13, 1899, 841; 

 Cladothrix caprae Mace, Traite Pratique 

 de Bact., 4th ed., 1901, 1094; Discomijces 

 caprae Gedoelst, Champ. Paras. Homme 

 et Anim., 1902, 174; Oospora caprae 

 Sartory, Champ. Paras. Homme et 

 Anim., 1923, 813; Actinomyces caprae 

 Ford, Textb. of Bact., 1927, 205.) From 

 Latin capra, goat. 



