930 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Description from Erikson (Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 26). 



Initial cells only slightly enlarged; 

 early development of aerial hyphae, 

 while substratum threads are still short ; 

 frequent slipping of branches ; aerial 

 mycelium abundant on all media with 

 tendency to form coherent spikes; my- 

 celium not very polymorphous, but 

 occasional thicker segments appear. 

 Slightly acid -fast. 



Gelatin: Extensive dull growth with 

 small raised patches of pink aerial myce- 

 lium; later ribbon-like, depressed. No 

 liquefaction. 



Glucose agar ; Irregular bright pink 

 growth tending to be heaped up; later 

 abundant masses frosted over with thin 

 white aerial mycelium. 



Glycerol agar : Abundant growth, small 

 round pink colonies, partly covered with 

 white aerial mycelium. 



Potato agar: Extensive thin growth, 

 pink in raised patches, covered by white 

 aerial mycelium; later aerial mycelium 

 also becomes pink. 



Starch agar : Minute colorless colonies 

 covered by white aerial mycelium. 



Blood agar : Minute round colorless 

 colonies aggregated in broad pink zones, 

 paler aerial mycelium. No hemolysis. 



Dorset's egg medium: Few colorless 

 colonies, some pink, white aerial myce- 

 lium; later, growth becoming dull pink, 

 irregular, with scant white aerial myce- 

 lium. 



Ca-agar: Minute colorless colonies, 

 white aerial mycelium; later a pinkish 

 tinge. 



Serum agar : Small round pink colonies 

 frosted over with thin white aerial my- 

 celium. 



Inspissated serum: No growth. 



Broth : Superficial pellicle composed of 

 pink colonies with white aerial mycelium ; 

 moderate flocculent sediment . 



Glucose broth : Small sediment of fine 

 flocculi ; later pellicle composed of small 

 pink colonies; superficial skin entire and 

 salmon-colored in 16 days. 



Synthetic glycerol solution : Round 

 pink disc-like colonies on surface and 

 tenuous white wispy growth in suspension 

 and sediment; after 20 days, surface 

 colonies bearing white aerial mycelium 

 extending 2 cm up tube. 



Synthetic sucrose solution : Minute 

 white colonies in suspension and sedi- 

 ment in 3 days; thin dust-like pellicle 

 in 10 days; some surface colonies with 

 white aerial mycelium in 17 days. 



Milk : Red surface skin ; solid coagulum. 



Litmus milk: Red surface growth, no 

 change in liquid; after 4 weeks, liquid 

 decolorized, opaque. 



Potato plug: Abundant growth, small 

 colonies, mostly confluent, entirely cov- 

 ered with pale pink aerial mycelium ; 

 growth becomes membranous, consider- 

 ably buckled ; later superficial colonies 

 with pink aerial mycelium on liquid at 

 base of tube, bottom growth of round 

 white colonies. 



Starch not hydrolyzed. 



Source: From lesions in goats. 



8. Nocardia pretoriana Pijper and 

 Pullinger. (Pijper and Pullinger, Jour. 

 Trop. Med. Hyg., 30, 1927, 153; Actino- 

 myces preiorianus Nannizzi, in Pollacci, 

 Tratt. Micopat. Umana, 4, 1934, 38.) 

 Named for Pretoria in South Africa. 



Description from Erikson. (Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 

 30). 



Minute fiat colonies are formed con- 

 sisting of angularly branched filaments, 

 and bearing a few short straight aerial 

 hyphae; later the growth becomes 

 spreading and extensive, the slipping of 

 the branches is well marked and the 

 aerial hyphae are divided into cylindrical 

 conidia. Slightly acid-fast. 



Gelatin: A few colorless flakes. No 

 liquefaction. 



Glucose agar : Pale buff umbilicated and 

 piled up colonies. 



Gl3^cerol agar: Piled up pink mass, 

 very scant white aerial mycelium at 

 margin. 



