FAMILY ACTINOMYCETACEAE 



901 



Ca-agar : Yellowish wrinkled coherent 

 growth with white aerial mycelium on 

 apices and at margin. 



Coon's agar: Colorless mostly sub- 

 merged growth, scant white aerial myce- 

 lium. 



Dorset's egg medium: A few round 

 colorless colonies in 3 days ; after 3 weeks, 

 irregular raised pink mass, warted ap- 

 pearance, moderate degree of liquefac- 

 tion. 



Serum agar: Raised, convoluted, 

 slightly pinkish growth. 



Inspissated serum: No growth. 



Broth: Moderate quantity of Hakes 

 and dust-like surface growth. 



Synthetic sucrose solution: A few 

 colorless flakes on the surface, lesser 

 bottom growth. 



Milk: Yellowish surface growth; solid 

 coagulum in one month; later, partly 

 digested, pale pink growth up the wall of 

 the tube. 



Litmus milk : Colorless surface growth, 

 liquid blue ; becoming hydrolyzed and 

 decolorized. 



Potato plug: Small raised pale pink 

 colonies with white aerial mj'celium; 

 after 2 months, plug and liquid dis- 

 colored, growth dull buff, dry and con- 

 voluted at base, round and zonate at top 

 of slant, white aerial mycelium, surface 

 and bottom growth on liquid. 



Source : From a case of mycetoma of 

 the chest wall in a South African native. 



Habitat : Human infectioas so far as 

 known. 



9. Nocardia pulmonalis (Burnett) 

 comb. nov. (Actinomyces pulmonalis 

 Burnett, Ann. Rept. N. Y. State Vet. 

 Coll., 1909-1910, 167.) From Latin 

 pulmo, lung. 



Gram-positive mj^celium breaking up 

 readily into oval-shaped conidia. Acid- 

 fast, especially in early stages of growth. 



Gelatin: Small, whitish, spherical 

 colonies ; edges of colony becoming chalky 

 white; limited liquefaction. 



Agar: Moist, raised growth in the form 

 of small, spherical colonies. 



Glucose agar: Dull, whitish, convo- 

 luted growth. 



Broth: Delicate, translucent film on 

 surface, becoming corrugated with some 

 whitish, spherical colonies in medium. 



Milk: Colonies on the surface of 

 the medium; milk is coagulated in a few 

 days, later digested. 



Potato : Lu.xuriant growth in the form 

 of small, translucent, round colonies; 

 becoming colored lemon-yellow; later, 

 growth becomes convoluted or folded 

 with chalky white aerial mycelium, color 

 of plug brownish. 



Non-pathogenic for rabbits and guinea 

 pigs. 



Aerobic . 



Source : From the lungs of a cow. 



Habitat: Bovine infections so far as 

 known . 



10. Nocardia parafBnae (Jensen) comb, 

 nov. {Proaclinomyces paraffinae Jensen, 

 Proc. Linn. Soc. New So. Wales, 56, 1931, 

 362.) From INI. L. paraffina, paraffine. 



In agar media, the organism initially 

 forms an extensive mycelium of long, 

 richly-branching hyphae, 0.4 to 0.5 mi- 

 cron thick. After 5 to 6 days, at room 

 temperature, numerous end branches 

 swell to about double thickness, become 

 more refractive, e.xhibit fine incisions 

 along their external contours, and divide 

 into oval, spore-like elements, 0.8 to 1.0 

 by 1.2 to 1.5 microns. This process of di- 

 vision starts at the tips of the swollen 

 branches and proceeds basipetally until 

 most of the hyphae appear divided. Pri- 

 mary septa have not been seen in the 

 hyphae. A similar process of division 

 takes place in liquid media, where also 

 the filaments often fall into fragments of 

 variable length. The spore-like elements, 

 but not the undivided filaments, are 

 markedly acid-fast. The aerial myce- 

 lium consists of rather short, straight, 

 not very much branched hyphae, 0.4 to 



