902 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



0.6 micron thick, which never show any 

 differentiation into spores. 



Gelatin: No liquefaction. 



Sucrose agar: Very scant growth. 

 Thin colorless veil, sometimes with a 

 trace of white aerial mycelium. 



Glucose agar: Fair growth. Vegeta- 

 tive mycelium flat, growing into me- 

 dium; pale ochre-yellow to orange, with 

 raised outgrowths on the surface. 

 Growth of a crumbly consistency. Scant, 

 white, aerial mycelium. 



Nutrient agar: Slow but good growth. 

 Vegetative mycelium superficial, some- 

 what raised, ochre-yellow, hard, but with 

 a loose, smeary surface. Aerial myce- 

 lium scant, small white tufts. No pig- 

 ment. 



Potato: Fair growth. Vegetative 

 mycelium granulated, first pale-yellow, 

 later deep ochre-yellow to orange. 

 Scant, white, aerial mycelium. No pig- 

 ment. 



Liquid media (milk, broth, synthetic 

 solutions) : Small, round granules of 

 various yellow to orange colors, firm, 

 but can be crushed into a homogeneous 

 smear. In old broth cultures a thick, 

 hard, orange to brownish surface pellicle 

 is formed. 



Sucrose not inverted. 



Starch not hydrolyzed. 



Cellulose is not decomposed. 



Nitrates are not reduced to nitrites. 



Milk is not coagulated or digested. 



Final reaction in glucose NH4CI solu- 

 tion, pH 4.6 to 4.4. 



All strains show a marked power of uti- 

 lizing paraffin wax as a source of energy. 



Source : Isolated from soil. 



Habitat : Soil. 



11. Nocardia minima (Jensen) comb, 

 nov. {Proactinomyces minimus Jensen, 

 Proc. Linnean Soc. New So. Wales, 56, 

 1931, 365.) From Latin minimus, very 

 small . 



Filaments and rods : 0.4 to 0.6 by 2 to 

 10 microns. In older cultures mostly 

 short rods, frequently V, Y, swollen 



forms, or cocci. Irregularly stained with 

 ordinary dyes, generally show bars and 

 bands. Generally a few cells from cul- 

 tures are acid-fast, most are not acid- 

 fast. Gram-positive. 



Gelatin stab : Filiform, granulated, 

 cream-colored growth. No liquefaction. 



Agar : Slow growth, raised, folded, with 

 finely myeloid margins. At first color- 

 less, after 6 to 8 weeks flesh pink or coral 

 pink. 



Potato : Growth slow, after 6 to 8 weeks 

 abundant, spreading, much raised, finely 

 wrinkled, coral pink. 



Paraffin is utilized. 



Optimum temperature 22° to 25°C. 



Distinctive characters : Closely re- 

 sembles Nocardia corallina but differs 

 in the much slower growth and the 

 smaller size of the cells. 



Source : From soil in Australia. 



Habitat: Soil. 



12. Nocardia corallina (Bergey et al.) 

 comb. nov. (Bacillus mycoides corallinus 

 Hefferan, Cent. f. Bakt., II Abt., 11, 

 1904, 459; Serratia corallina Bergey et 

 al., Manual, 1st ed., 1923, 9Z;Streptothrix 

 corallinus Reader, Jour. Path, and Bact., 

 29, 1926, 1 ; Mycobacterium agreste Gray 

 and Thornton, Cent. f. Bakt., II Abt., 

 73, 1928, 84; Actinomyces agrestis Bergey 

 et al., Manual, 3rd ed., 1930, 472; Pro- 

 actinomyces agrestis Jensen, Proc. Lin- 

 nean Soc. New So. Wales, 56, 1931, 345; 

 Proactinomyces corallinus Jensen, ibid., 

 57, 1932, 364.) From Latin corallinus, 

 coral red. 



Description from Gray and Thornton 

 {loc. cit.), Jensen {loc. cit.) and Bynoe 

 (Thesis, McGill University, Montreal, 

 1931). 



Branching rods, generally curved, 1 to 

 1.5 by 3 to 10 microns. In older cultures 

 generally shorter rods and cocci. Non- 

 motile. Not acid-fast. Gram-positive. 



Gelatin colonies : Round, convex, 

 smooth, pink, shining, edge filamen- 

 tous. Deep colonies: Burrs. 



