904 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



ducing a more watery type of surface 

 growth, more nearly entire deep colonies 

 and more particularly by the production 

 of indigotin from indol. 



Source: From soil in Great Britain. 



Habitat: Presumably soil. 



14. Nocardia salmonicolor (den Dooren 

 de Jong) comb. nor. {M ycobacterium 

 salmonicolor den Dooren de Jong, Cent, 

 f. Bakt., II Abt., 71, 1927, 216; Flavo- 

 hacterium salmonicolor Bergey et al., 

 Manual, 3rd ed., 1930, 157; Proactino- 

 myces salmonicolor Jensen, Proc. Linnean 

 Soc. New So. Wales, 57, 1932, 368.) 

 From Latin salmo, salmon and color, 

 color. 



Closely related to Nocardia corallina. 



On glucose -as paragine-agar after 18 to 

 24 hrs., long branching rods are formed, 

 1.0 to 1.3 microns in thickness, with 

 small refractive granules of aerial my- 

 celium, sometimes stretching into quite 

 long filaments; after 2 to 3 days small 

 definite mycelia are present, and after 5 

 to 6 days these have largely divided into 

 short rods and cocci; the colonies have 

 the same burr-like appearance as those 

 of Nocardia corallina. Many cells at 

 the edge of the colonies show, after 3 to 



4 days, club- or pear-shaped swellings, 

 up to 2.5 to 3.0 microns in width; after 



5 to 6 days, many of these swollen cells 

 are seen to germinate with the formation 

 of two more slender sprouts. {0rskov, 

 Investigations into the Morphology of 

 the ray fungi. Copenhagen, 1923, 82, 

 gives an almost identical picture of 

 Streptothrix rubra; it is questionable, 

 indeed, whether these two organisms 

 are not really identical.) 



Gelatin: At 20'' to 22°C, scant arbores- 

 cent growth in stab; small wrinkled 

 orange surface colony. No liquefaction. 



Glucose-asparagine-agar : Good growth, 

 restricted, rather flat, edges lobate, sur- 

 face warty, glistening, first pale orange, 

 later ochre-yellow; consistency crumbly. 

 After 5 to 6 weeks the growth is paler 



with manj' small round raised j'ellow 

 secondary colonies. 



Glucose-nutrient agar: Excellent 

 growth, spreading, fiat, dense, edges lo- 

 bate, surface folded, glistening, yellow, 

 gradually changing to deep orange-red. 



Nutrient broth : Fair growth ; thin pel- 

 licle and granular sediment, at first 

 cream-colored, later red; broth clear at 

 first, slightly turbid after 3 w'eeks. 



Milk : Good growth ; pellicle of small 

 cream-colored granules after 2 days, later 

 a thick orange sediment. Not coagu- 

 lated, but appears slightly cleared after 

 5 weeks, the reaction becoming alkaline. 



Potato. Good growth, raised, warty, 

 crumbly, glistening, at first buff, chang- 

 ing to orange and finally to almost blood - 

 red. 



Indole not formed. 



Nitrites produced from nitrates. 



Nitrate, ammonium salts, asparagine 

 and peptone are utilized almost equally 

 well with glucose as source of carbon, 

 although the growth is most rapid with 

 peptone. 



Sucrose not inverted, although readily 

 utilized with sodium nitrate as a source 

 of nitrogen. 



Paraffin readily utilized as a source of 

 carbon . 



Phenol not utilized. 



No acid from glucose or glycerol. 



Starch not hydrolyzed. 



No growth in oxygen-free atmosphere. 



Source : Isolated from soil by means of 

 an ethylamine enriched medium, at 37°C. 



Habitat: Probably soil. 



15. Nocardia rubropertincta (Heffer- 

 an) comb. nov. (Butterbacillus, Grass- 

 berger, Mi'inch. med. Wochnschr., 46, 

 1899, 343; Bacillus rubropertinctus Hef- 

 feran. Cent. f. Bakt., II Abt., 11, 1903, 

 460; Serratia rubropertinctus Bergey et 

 al.. Manual, 1st ed., 1923, 96; Mycobac- 

 terium rubropertinctum Ford, Textb. of 

 Bact., 1927, 255; Proactinomyces ru- 

 bropertinctus Reed, in Manual, 5th ed.. 



