906 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Mycelium produced at first but soon 

 breaks up into rods and cocci . The latter 

 multiply by fission, cross-wall formation 

 and budding. Cells are Gram-positive, 

 weakljr acid-fast. 



Gelatin : No liquefaction. 



Colonies smooth or folded and rough, 

 shiny or dull, dark red color. Pigment 

 belongs to the carotinoids, does not 

 diffuse into the medium. 



Milk: No coagulation or peptonization. 



Sucrose not inverted. 



Starch not hydrolyzed. 



No growth on cellulose. 



Readily assimilates fats and paraffin, 

 and, to a less extent, wax. 



Various strains of this organism may 

 vary considerably from type. 



Habitat : Soil. 



17. Nocardia coeliaca (Gray and 

 Thornton) comb. nov. {Mycohactcriuni 

 coeliacum Gray and Thornton, Cent. f. 

 Bakt., II Abt., 73, 1928, 88; Flavobacie- 

 rium coeliacum Bergey et al., Manual. 

 3rd ed., 1930, 156; Proactinomyces co- 

 eliacus Reed, in Manual, 5th ed., 19-39, 

 836.) 



Description from Gray and Thornton 

 {loc. cil.) and from Jensen (Proc. Lin- 

 nean Soc. New So. Wales, 56, 1931, 201). 



Short, curved, uneven-sided rods : 0.8 

 by 5 microns with occasional filaments up 

 to 10 to 12 microns long, frequently 

 beaded, occasionally swollen or branched. 

 Coccoid forms 0.8 to 1.2 microns in di- 

 ameter are common, especially in older 

 cultures. Stain readily. Not acid -fast 

 or occasionally slightly acid-fast . Gram- 

 positive. 



Gelatin colonies : After 12 days, irregu- 

 lar, raised, white, rugose, dull, edge en- 

 tire. Deep colonies : Irregular, smooth or 

 slightly broken. 



Gelatin stab: Convoluted, buff-white 

 to yellowish, dull; below surface the 

 growth forms many irregular hollow lobes, 

 giving a glistening appearance, to a depth 

 of 3 to 4 mm. 



Agar colonies: After 11 days, less than 



1 mm in diameter, round or irregular, 

 raised, white, resinous, edge irregular, 

 burred. Deep colonies : Irregularly 

 round or oval, edge slightly broken. 



Agar slant : Filiform, convex, white, 

 rugose, resinous, edge undulate. 



Broth: Turbid. 



Litmus milk: Slightly alkaline after 5 

 to 7 days. 



Glycerol potato: After 2 days, dry, 

 crumpled, orange, becoming brown after 

 about 10 da5^s. 



Dorset's egg medium: Raised, smooth, 

 moist, verrucose, buff-colored. 



Loeffler's medium: After 10 days, 

 slight growth, dry, granular, pale buff- 

 colored. 



Nitrites are not produced from nitrates. 



No acid from glucose, lactose, sucrose 

 or glj'cerol. 



Phenol is utilized. 



Optimum pPI 7.6 to 8.0. 



Optimum temperature 22° to 25°C. 



Distinctive characters : Differs from 

 the previously described members of the 

 genus in the absence of chromogenesis. 

 Forms hollow lobes in deep gelatin cul- 

 tures. Cells are rods, seldom filaments. 



Source : From soil in Great Britain and 

 Australia. 



Habitat : Presumably soil. 



IS. Nocardia transvalensis Pijper and 

 Pullinger. (Pijper and Pullinger, Jour. 

 Trop. Med. Hyg., 30, 1927, 153; Ac- 

 tinomyces transvalensis Nannizzi, in 

 Pollacci, Tratt. Micopat. Umana, 4, 

 1934, 46.) Named for the Transvaal, a 

 state of South Africa. 



Description from Erikson (Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 

 28). 



Initial mycelium unicellular, but with 

 the central branch frequently broader 

 and showing dense granular refractile 

 contents, small colonies quickly covered 

 with aerial mycelium, the straight aerial 

 hyphae in some cases becoming clustered 

 into irregular spikes, colorless drops are 

 exuded and a pink coloration produced in 



