908 



MAMAL OF DETEKMlAATiVK BACTERIOLOGY 



Nitrogen is utilized as sodium nitrate, 

 ammonium phosphate and asparagine, 

 although tliese are inferior to peptone as 

 sources of nitrogen. 



Source: Fermented beets. 



20. Nocardia flava (Krassilnikov) 

 comb. tiov. (Proactinoniyces flavus Kras- 

 silnikov, Bull. Acad. Sci. U. S. S. R., 

 No. 1, 1938, 139.) From Latin flavus, 

 yellow. 



Cells at first filamentous, 0.7 to 0.8 

 micron in diameter; after 2 to 3 days 

 broken into long rods and then into 

 cocci 0.7 micron in diameter. No spores, 

 some strains form chlamydospores. Cell 

 multiplication by fission, cross-wall for- 

 mation, rarely by budding. Cells 

 Gram-positive; not acid-fast. 



Gelatin: No liquefaction. 



Synthetic agar colonies : Bright yellow 

 or gold color. 



Meat peptone media : Dirty yellow 

 pigmentation. 



Agar colonies : Pigment bright yellow 

 or gold color on synthetic media, dirty 

 yellow on meat peptone media. Pigment 

 not soluble in medium. Surface of 

 colony somewhat shiny or rough and 

 folded, of a dough-like consistency. 



Milk: No peptonization or coagulation. 



Sucrose weakly inverted. 



Starch is hydrolyzed. 



Does not grow on paraffin and wax but 

 makes weak growth on fat. 



Habitat : Soil, not common. 



21. Nocardia viridis (Krassilnikov) 

 comh. 710V. {Proaclinomyces viridis 

 Krassilnikov, Bull. Acad. Sci. U. S. S. R., 

 No. 1, 1938, 139). From Latin viridis, 

 green. 



Mycelial cells often branching, 0.7 to 

 0.8 micron in diameter with cross-wall; 

 after 5 to 7 days the cells break up into 

 rods 5 to 15 microns long. Cocci not 

 observed. Cells multiply by fission, 

 seldom by budding. Spores not formed. 

 Cells Gram-positive, not acid-fast. 



Gelatin: No liquefaction. 



Colonies colored dark green. Pigment 

 not soluble in medium, in water or in 

 organic solvents. Surface of colony 

 somewhat shiny. On potato, rough, 

 much folded, broken up into small col- 

 onies. 



Milk: No peptonization or coagulation. 



Sucrose readily inverted. 



Starch weakly hydrolj^zed. 



Grows well on fats and paraffin and 

 less on wax. 



Habitat: Soil. 



22. Nocardia citrea (Krassilnikov) 

 comb. nov. {Proaclinomyces citrous 

 Krassilnikov, Bull. Acad. Sci. U. S. S. R., 

 No. 1, 1938, 139.) From M. L. citreus, 

 lemon yellow. 



Mycelium in young cultures consists 

 of ver3' fine threads 0.3 to 0.5 micron in 

 diameter. After several days the cells 

 break up into short rods 0.5 by 1.5 to 5 

 microns and into cocci 0.3 to 0.5 micron 

 in diameter. Multiplies by fission and 

 bud formation ; spores not formed. Cells 

 not acid -fast. 



Gelatin: Liquefaction. 



Colonies: Yellow -green, usuallj^ rough 

 and folded. 



Milk: Coagulation and peptonization. 



Sucrose is inverted. 



Starch is hydrolyzed. 



Weak growth on fat. No growth on 

 paraffin or wax. 



Habitat : Soil and water. 



23. Nocardia madurae (Vincent) 

 Blanchard. {Streptothrix madurae Vin- 

 cent, Ann. Inst. Past., 8, 1894, 129; 

 Blanchard, Bouchard's Traite de Path. 

 Gen., 2, 1896, 868; Oospora madurae 

 Lehmann and Neumann, Bakt. Diag., 

 1 Aufl., 2, 1896, 388; Actinomyces madurae 

 Lachner-Sandoval, Ueber Strahlenpilze, 

 1898, 64; Cladothrix madurae Mace, 

 Trait<5 Pratique de Bact., 4th ed., 1901, 

 1090; Discomyces madurae Gedoelst, 

 Champ. Pai'as. Homme et Animaux, 

 Paris. 1902. 169. ) Named for the disease 



