FAMILY ACTINOMYCETACEAE 



909 



Madura foot, with which this organism 

 is associated. 



Oospora indica Kant hack (Ivanthack, 

 Jour. Path, and Bact., /, 1893, 140; No- 

 cardia indica Chalmers and Christo- 

 pherson, Ann. Trop. Med. and Parasit., 

 10, 1916, 231 ; Discomyces indicus Neveu- 

 Lemaire, Precis de Parasitol. Hum., 

 5th ed., 1921, 42; Actinomyces indicus 

 Brumpt, Precis de Parasitol., Paris, 

 4th ed., 1927, 1196) is regarded by some 

 authors as identical with Nocardia 

 madurae Blanchard. If this is estab- 

 lished, then the correct name of the 

 organism is Nocardia indica (Kanthack) 

 Chalmers and Christopherson. 



The species described under the name 

 Actinomyces madurae in previous editions 

 of Bergey's Manual is definitely not the 

 true causative agent of the disease and is 

 probably a contaminant carried as a cul- 

 ture of this species. 



Morphology in tissues, growth in form 

 of granules consisting of radiating 

 actinomycosis. In cultures, initial 

 branched mycelium fragmenting into 

 rod -shaped and coccoid bodies. No 

 aerial mycelium or spores. Not acid- 

 fast. 



Gelatin: Growth scant, whitish; no 

 liquefaction. 



Gelatin colonies : Round, glistening, at 

 first white, then buff to rose or crimson. 

 Pigment production is irregular and un- 

 predictable. Occasionally red soluble 

 pigment is produced. Growth eventu- 

 ally wrinkled. No aerial mycelium. 



Potato: Wrinkled friable growth; buff- 

 colored, sometimes red. 



Broth : Growth as a floccular sediment. 



Milk: No change, or slight slow pep- 

 tonization. 



Diastatic (?) action. 



Not pathogenic for the usual laboratory 

 animals; pathogenic for monkeys (Mus- 

 grave and Clegg, Philippine Jour. Sci., 

 Ser. B., Med. Sci., 3, 1908, 470). 



Habitat : Cause of some cases of Madura 

 foot. 



24. Nocardia lutea Christopherson and 

 Archibald. (Christopherson and Archi- 

 bald, Lancet, 2, 1918, 847; Actinomyces 

 luteus Brumpt, Precis de Parasitol., 

 Paris, 4th ed., 1927, 1206.) From Latin 

 luteus, yellow. 



Description from Erikson (Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 30). 



Initial elements swollen and seg- 

 mented, giving rise to irregular spreading 

 polymorphous colonies composed of cells 

 of all shapes and sizes with markedly 

 granular contents. Later more mono- 

 morphous, the filaments being arranged 

 in angular apposition. Sometimes (e.g., 

 on synthetic glycerol agar) the segments 

 are so granular as to appear banded. On 

 potato agar, small filamentous colonies 

 are formed with irregular angular branch- 

 ing and bear a few isolated short straight 

 aerial hyphae. 



Gelatin : Pale pink wrinkled growth on 

 wall of tube and colorless punctiform and 

 stellate colonies in medium ; no liquefac- 

 tion. 



Agar: Abundant, coherent, moist, pink 

 membranous growth with round discrete 

 colonies at margin ; after 3 weeks colorless 

 fringed margin, round confluent portion. 



Glucose agar : Scant reddish smeary 

 growth. 



Glycerol agar : Yellowish-pink, 

 wrinkled membrane. 



Potato agar : Coherent pink moist 

 growth, centrally embedded with small 

 round discrete colonies at margin. 



Dorset's egg medium: Poor growth, 

 dull pink, spreading. 



Serum agar : Confluent granular pink 

 membrane. 



Broth : Pink flakes and surface growth. 



Inspissated serum : Raised convoluted 

 pink mass; becoming orange, much 

 wrinkled, scalloped margin. 



Synthetic sucrose solution : Red 

 granules and abundant minute colorless 

 colonies at bottom ; in 2 weeks a colorless 

 dust-like surface pellicle. 



Glucose broth : Abundant, pinkish 



