910 



]VL\NUAL OF DETERMINATIVE BACTERIOLOGY 



flaky surface growth, breaking up easily 

 and sinking to bottom. 



Litmus milk: Orange-red surface and 

 bottom growth, liquid blue. 



Potato plug: Carrot-red, moist, thick, 

 granular growth in bands, partly raised 

 and with discrete round colonies ; sparse 

 colorless very thin aerial mycelium at 

 top of slant in 2 months. 



Source : From actinomycosis of the 

 lachrymal gland. 



F 25. Nocardia blackwellii (Erikson) 

 comb. nov. {Actinomyces blackwellii Erik- 

 son, Med. Res. Counc. Spec. Rept. Ser. 

 203, 1935, 37.) 



Description from Erikson {loc. cit., 

 p. 32). 



Initial elements short, rod-like, grow- 

 ing out into longer forms sparsely branch- 

 ing; small radiating colonies are produced 

 with short straight aerial mycelium, 

 frequently large round or ovoid cells are 

 interposed in the irregularly segmented 

 chains of cells, being sometimes isolated 

 in company with 2 or 3 short filaments 

 and sometimes terminal. 



Gelatin : Few colorless minute colonies 

 along line of inoculation; after 30 days 

 abundant colorless colonies to 10 mm be- 

 low surface, larger pink-yellow surface 

 colonies with white aerial mycelium; no 

 liquefaction. 



Agar : Confluent wrinkled growth with 

 small, round, pinkish, discrete colonies 

 at margin. 



Glucose agar: Abundant, pale pink 

 growth, small conical colonies, piled up, 

 convoluted. 



Glycerol agar : Extensive, granular, ir- 

 regular, thin, pinkish growth; after 40 

 days, a few discrete colonies with de- 

 pressed margins, center piled up, pink. 



Serum agar: Smooth, cream, umbili- 

 cated colonies, with submerged growth 

 extending into medium in scallops 5 to 8 

 mm deep; a pale pink mass in 2 weeks, 

 t Potato agar: Small, round, colorless 

 colonies covered with white aerial my- 

 celium; after 2 weeks colonies dull pink, 



submerged margins, few aerial spikes, 

 moderate aerial mycelium at top of 

 slant. 



Broth: Flakes, later innumerable 

 minute colonies, some adhering to wall 

 just above liquid level. 



Synthetic sucrose solution : Delicate, 

 round, white colonies ; later abundant 

 minute colonies in suspension, thick 

 cream pellicle on surface and pink 

 grains in sediment. 



Milk : Heav}' convoluted bright j^ellow 

 surface pellicle, no coagulation. 



Litmus milk: Yellow surface growth, 

 nrdlky sediment, liquid unchanged. 



Carrot plug: Small, round, smooth, 

 cream-colored elevated colonies in 10 

 days; sparse stiff colorless aerial spikes 

 in 16 da3\s ; abundantly piled up, con- 

 voluted, ochreous growth in 25 days. 



Source : From hock joint of foal. 



26. Nocardia cuniculi Snijders. 

 (Snijders, Geneesk. Tijdsch. Med. Ind., 

 64, 1924, 47 and 75; Aclinoviyces cuniculi 

 Erikson, Med. Res. Council Spec. Rept. 

 Ser. 203, 1935, 32; not Actinomyces 

 cuniculi Gasperini, Cent. f. Bakt., 15, 

 1894, 684; Actinomj/ces sumatrae Erikson, 

 loc. cit., 32.) From Latin cuniculus, 

 rabbit. 



Description taken from Erikson {loc. 

 cit., p. 31). 



Large swollen cells give rise to ramify- 

 ing filaments or to small chains of short 

 thick segments which branch out into 

 more regular hyphae; sometimes the ir- 

 regular elements are beset with spiny 

 processes before giving rise to typical 

 long branching filaments ; later the pic- 

 ture becomes more monomorphous and 

 short straight aerial hyphae are borne, 

 which presently exhibit irregular seg- 

 mentation. 



Gelatin: Few flakes. No liquefaction. 



Agar: Small, round, elevated, cream- 

 colored colonies, umbilical ed and radially 

 wrinkled. 



Glucose agar: Minute, colorless colon- 



