FAMILY ACTINOMYCETACEAE 



911 



ies ; becoming dull pink, partly confluent 

 and piled up, fewstiif pink aerial spikes. 



Glycerol agar: Small round elevated 

 cream-colored colonies, margins de- 

 pressed; becoming smooth, discrete, 

 yellowish. 



Dorset's egg medium: Scant pinkish 

 smeary growi;h. 



Serum agar : Small, raised, cream- 

 colored colonies, becoming confluent and 

 piled up. 



Inspissated serum: Thick, colorless, 

 ribbed membrane; no liquefaction. 



Broth : Small and larger cream-colored, 

 scale-like surface colonies, abundant, 

 flocculent bottom growth. 



Sj'nthetic sucrose solution; Thin sur- 

 face pellicle, small colorless flakes, 

 minute particles at bottom, scant growth. 



Milk : Heavj^ yellow growth attached to 

 walls; solid coagulum in 1 month. 



Litmus milk : Yellow surface growth, 

 liquid unchanged. 



Potato plug: Coral-pink, dry, granular 

 growth, covered to a considerable extent 

 with white aerial mycelium, piled up in 

 center, discrete colonies at margin, pink 

 surface pellicle on liquid and colorless 

 colonies at base. 



Source: Infected rabbits. 



27. Nocardia rangoonensis (Erikson) 

 comb. nov. {Actinomyces rangoon Erik- 

 son, Med. Res. Council Spec. Rept. Ser. 

 203, 1935, 37.) 



Description from Erikson (lac. cit., 

 p. 33). 



Swollen round initial cells, giving rise 

 to branching hyphae which segment and 

 present slipping and angular arrange- 

 ment; few short straight aerial hyphae, 

 which later develop into a profusely 

 branching long waving aerial mycelium. 

 Non-acid-fast. 



Gelatin: Abundant minute colonies in 

 depths and larger cream-colored ones on 

 surface with white aerial mycelium; 

 brown pigment surrounding growth. No 

 liquefaction. 



Agar colonies : Round, lobate, umbili- 



cated, raised up, cream-colored to pale 

 pink; later, medium discolored dark 

 brown, colonies colorless. 



Glucose agar : Convoluted, coherent, 

 cream-colored growth, medium dis- 

 colored. After 23 days, wrinkled, bis- 

 cuit-colored growth, colorless margin, 

 border white aerial mycelium, medium 

 dark brown. 



Glycerol agar: Dull, mealy, pink, 

 wrinkled growth, scant white aerial myce- 

 lium at top, medium slightly discolored. 



Coon's agar : Minute colorless colonies 

 in streaks. 



Potato agar: Small, round, lemon- 

 colored colonies, parth'' confluent, with 

 white aerial mycelium; later medium dis- 

 colored light brown; submerged grov\i;h 

 greenish. 



Dorset's egg medium: Extensive color- 

 less growth, pale pink aerial mycelium in 

 center; later covered with a powdery 

 pinkish-white aerial mycelium. 



Serum agar colonies : Irregular, small, 

 elevated, cream-colored, frequently um- 

 bilicated. 



Inspissated serum: Poor growth, small 

 piled up pink mass . 



Broth: Abundant colorless growth, 

 flocculent mass at bottom and pellicle 

 at surface, medium slightly discolored. 



Synthetic sucrose solution : Small white 

 colonies with pinkish tinge on surface, 

 lesser bottom growth. 



Milk : Coagulation, j^ellow surface ring, 

 becoming partly peptonized, liquid dis- 

 colored dark brown, brownish growth up 

 side of tube. 



Litmus milk: Colorless growth, liquid 

 partly decolorized; coagulation; later 

 partly digested. 



Carrot plug: Small round colorless 

 colonies, velvety white aerial mycelium; 

 in 2 months, piled up pink granular mass 

 with warted prominences, marginal zone 

 white aerial mycelium and thin all-over 

 central aerial mycelium. 



Source : Human pulmonary case of 

 streptothricosis. 



