912 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Habitat : Human infections so far as 

 known. 



28. Nocardia caviae Snijders. 



(Snidjers, Geneesk. Tijdschr. Ned. Ind., 

 64, 1924, 47 and 75; Actinomyces caviae 

 Erikson, Med. Res. Council Spec. Rept. 

 Ser. 203, 1935, 37.) From the generic 

 name of the guinea pig. 



Description from Erikson {loc. cit., 

 p. 32). 



Initial segmentation, producing ele- 

 ments of appro.ximately even thickness 

 arranged in angular apposition, and later 

 long profusely ramifying threads with 

 strongly refractile protoplasm. Aerial 

 mycelium straight and branching, the 

 aerial hyphae with occasional coiled tips 

 divided into cylindrical conidia. 



Gelatin: A few colorless flakes. No 

 liquefaction. 



Glucose agar: Piled up, convoluted, 

 cream-colored to pale pink growth, white 

 aerial mycelium. 



Glycerol agar: Scanty growth. 



Coon's agar: Colorless scant growth, 

 partly submerged, white aerial mycelium. 



Potato agar : Colorless spreading growth 

 with dense white aerial mycelium. 



Dorset's egg medium: Heavily corru- 

 gated pale pink growth with submerged 

 margin, dense white aerial mycelium in 

 center ; after 3 weeks, colorless transpired 

 drops. 



Serum agar: Pale pink wrinkled 

 growth, partly submerged; after 4 weeks, 

 piled up with scant white aerial myce- 

 lium, medium discolored reddish-brown. 



Inspissated serum : Pale pink raised 

 growth, coiled, white aerial mycelium. 



Broth : Cream-colored wrinkled surface 

 pellicle extending up wall and breaking 

 easily, moderate bottom growth, flaky, 

 medium discolored. 



Synthetic sucrose solution: Round 

 white colonies in suspension and attached 

 to one side of tube, pink surface colonies 

 with white aerial mycelium. 



Milk: Colorless surface growth, white 

 aerial mycelium; coagulation. 



Litmus milk: Liquid blue, surface 

 growth; after 1 month, white aerial my- 

 celium, colorless sediment, liquid still 

 blue. 



Potato plug: Small colorless colonies, 

 white powdery aerial mycelium; later 

 abundant raised pale pink confluent 

 growth, discolored plug; after 2 months, 

 raised buckled pink colonies with white 

 aerial mycelium floating on liquid at base. 



Source : Infected guinea pigs, Sumatra. 



29. Nocardia actinomorpha (Gray and 

 Thornton) comb. nov. {Mycobacterium 

 actinomorphum Gray and Thornton, Cent, 

 f. Bakt., II Abt., 73, 1928, 88; Actino- 

 myces actinomorphus Bergey et al., 

 Manual, 3rd ed., 1930, 471; Proactino- 

 myces actinomorphus Jensen, Proc. Lin- 

 nean Soc. New So. Wales, 56, 1931, 363.) 

 From Greek actis, ray and Latin morpha, 

 shape, form. 



Description from Gray and Thornton 

 (loc. cit.), Jensen {loc. cit.), and Bynoe 

 (Thesis, McGill University, Montreal, 

 1931). 



Long branching filaments and rods : 0.5 

 to 0.8 by up to 10 microns. In older cul- 

 tures rods 2 to 3 microns long generally 

 predominate. On some media exten- 

 tively branching hyphae occur. Readily 

 stained. Not acid-fast. Gram-positive. 



Gelatin colonies : After 12 days, round, 

 saucer-like, white, raised rim, edges 

 burred. Liquefaction. Deep colonies: 

 Burrs. 



Gelatin stab : After 8 to 14 days, sac- 

 cate liquefaction, 5 to 8 mm. 



Agar colonies: After 11 days, round, 1 

 mm in diameter, convex, white, granular 

 or resinous ; long arborescent processes 

 from the edge. Deep colonies: Arbores- 

 cent burrs ; processes about equal to 

 diameter of colony. 



Agar slant : Filiform, raised to convex, 

 white, rugose, dull; edge undulate, with 

 strong tufted projections below surface. 



Broth: Turbid, or clear with white 



