936 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Aerobic. 



Distinctive character : Litmus-like pig- 

 ment. 



Source : Dust contamination on a 

 potato slant. 



Habitat: Soil and plant surfaces. 

 Very abundant. 



Note : Because of the numerous colors 

 and shades shown by the pigment ac- 

 cording to final H-ion concentration and 

 other less understood factors, this species 

 may have been described under various 

 names. On the other hand, it is entirely 

 possible, as pointed out by Conn {loc. 

 cit.), that careful study of the pigments 

 may show that more than one species is 

 actually involved. 



3. Streptomyces verne (Waksman and 

 Curtis) comb. nov. (Actino^nyces verne 

 Waksman and Curtis, Soil Science, 1, 

 1916, 120.) Derivation uncertain. 



Filaments with close branching of the 

 hyphae. No conidia demonstrated. 



Gelatin stab: Small, cream-colored 

 colonies. Rapid liquefaction. 



Synthetic agar: Abundant, spreading, 

 wrinkled, elevated, glossy, yellowish 

 growth, becoming brownish, lichenoid 

 margin. 



Starch agar: Scant, brownish, re- 

 stricted growth. 



Glucose agar: Abundant, much folded 

 growth, center raised, gray with purplish 

 tinge, entire. 



Plain agar : Small, grayish colonies with 

 depressed center, becoming wrinkled. 



Glucose broth: Slightly flaky sediment. 



Litmus milk: Pinkish-brown ring; 

 coagulated; peptonized, with alkaline 

 reaction. 



Potato : Cream-colored growth, be- 

 coming gray, wrinkled. 



Nitrites produced from nitrates. 



Soluble brown pigment formed. Sol- 

 uble green pigment produced when 

 freshly isolated. 



Starch is hydrolyzed. 



Aerobic . 



Optimum temperature 37°C. 



Source : Isolated once from upland 

 California soil. 

 Habitat: Soil. 



4. Streptomyces californicus (Waks- 

 man and Curtis) comb. nov. (Actinomyces 

 californicus Waksman and Curtis, Soil 

 Science, 1, 1916, 122.) Named for the 

 State of California. 



Filaments with long, narrow, open 

 spirals. Spherical to oval conidia from 

 straight and spiral hyphae. 



Gelatin stab : Gray, moist, abundant 

 surface growth. Liquefaction in 30 days. 



Synthetic agar: Spreading, vinaceous- 

 colored growth. Aerial mycelium pow- 

 dery, thin, light neutral gray. 



Starch agar: Growth spreading, pink 

 center with colorless to gray margin. 



Glucose agar : Restricted, much folded, 

 cream-colored growth, with sulfur-yellow 

 tinge. 



Plain agar: Thin, restricted, yellowish 

 to cream-colored growth. 



Glucose broth ; Solid cream-colored 

 mass on surface, with pink tinge. 



Litmus milk: Faint, brownish surface 

 growth; coagulated; peptonized in 40 

 days. 



Potato: Glossy, yellow to red growth, 

 turning red-brown. 



Nitrites produced from nitrates. 



No soluble pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 37°C. 



Source : Isolated once from California 

 sandy loam. 



Habitat: Soil. 



5. Streptomyces flaveolus (Waksman) 

 comb. nov. (Actinomyces 168, Waksman, 

 Soil Science, 8, 1919, 134; Actinomyces 

 flaveolus Waksman, in Manual, 1st ed., 

 1923, 368; Actinomyces heimi Duchd, 

 Actinomyces du groupe albus. Encyclo- 

 pedic Mycologique, Paris, 6, 1934, 359.) 

 From Latin flavus, yellow and eolus, 

 diminutive ending; hence, somewhat 

 yellow. 



