938 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Optimum temperature 37°C. 

 Habitat : Soil . 



8. Streptomyces griseolus (Waksman) 

 comb. not'. (Actinomyces 96, Waksman, 

 Soil Science, 8, 1919, 121 ; Actinomyces 

 griseolus Waksman, in Manual, 1st eel., 

 1923, 369.) From Latin griseus, gray and 

 eolus, diminutive ending; hence, some- 

 what gray. 



Branching mycelium; no spirals ob- 

 served. Conidia spherical or oval- 

 shaped. 



Gelatin stab : Liquefied with yellowish, 

 flaky pellicle and sediment. 



Synthetic agar : Colorless, thin, spread- 

 ing growth, chiefly in the medium; sur- 

 face growth limited almost entirely to the 

 aerial mycelium. Aerial mycelium at 

 first gray, later becoming pallid, neutral - 

 gray. 



Starch agar: Grayish-brown growth, 

 with dark ring. 



Glucose agar: Spreading growth, both 

 on the surface and into the medium; 

 center raised, cream-colored, turning 

 dark. 



Plain agar: Brownish growth, with 

 smooth surface. 



Glucose broth : Thick, brown ring. 



Litmus milk: Abundant growth, pink 

 pellicle; coagulated; peptonized, becom- 

 ing alkaline. 



Potato: Cream-colored growth, becom- 

 ing black, spreading. 



Nitrites produced from nitrates. 



Faint brownish soluble pigment 

 formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Habitat: Soil. 



9. Streptomyces erythreus (Waksman) 

 comb. nov. (Actinomyces 161, Waksman, 

 Soil Science, 8, 1919, 112; Actinomyces 

 erythreus Waksman, in Manual, 1st ed., 

 1923, 370; Actinomyces krainskii Duchd, 

 Encyclopedic Mycologique, Paris, G, 

 1934, 306.) From Greek erythrus, red. 



Mycelium fine, branching; numerous 

 open spirals formed as side branches of 

 the main hyphae. 



Gelatin stab : Abundant, dense, gray 

 growth with pinkish tinge, chiefly on sur- 

 face of liquefied medium. 



Synthetic agar: Spreading growth with 

 irregular margin, developing deep into 

 the medium; color at first white, later 

 turning yellowish, agar around growth 

 has a white, milky surface. Aerial myce- 

 lium, thick, solid, white. 



Starch agar: Cream-colored, circular 

 colonies, with faint greenish tinge. 



Glucose agar: Abundant, spreading, 

 cream-colored growth, later turning 

 brown chiefly on surface ; center raised, 

 lobate margin. 



Plain agar: Cream-colored growth. 



Glucose broth: Abundant, cream- 

 colored surface growth. 



Litmus milk : Yellowish surface zone ; 

 coagulated; peptonized, becoming alka- 

 line. 



Potato : Wrinkled, cream-colored 

 growth, becoming yellowish. 



Nitrites produced from nitrates. 



Soluble purple pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Similar to Streptomyces erythrochromo- 

 genes (Species No. 24) except that no 

 brown soluble pigment is formed. 



Source : From California and Hawaiian 

 soils. 



Habitat: Soil. 



10. Streptomyces cellulosae (Krain- 

 sky) comb. nov. {Actinomyces cellulosae 

 Krainsky, Cent. f. Bakt., II Abt., U, 

 1914, 662.) From M. L. cellulosa, cellu- 

 lose. 



Conidia almost spherical, 1.3 microns 

 in diameter, often arranged in chains. 



Gelatin colonies: Circular, j'ellowish. 



Gelatin stab: Liquefied. 



Plain agar: White aerial mycelium. 



Ca-malate agar: Yellowish colonies; 



