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MANUAL OF DETERMINATIVE BACTERIOLOGY 



growth. Aerial mj'celium white, becom- 

 ing drab gray. 



Starch agar: Thin, colorless, spreading 

 growth. Aerial mycelium gray. 



Glucose agar : Yellowish, spreading 

 growth. No aerial mycelium. 



Plain agar: Cream-colored growth. 

 Thin aerial mycelium. 



Glucose broth: Gray ring with grayish 

 colonies in bottom of tube. 



Litmus milk: Brownish ring; coagu- 

 lated ; peptonized in 25 to 30 days, becom- 

 ing faintly alkaline. 



Potato: Abundant, wrinkled, cream- 

 colored growth with greenish tinge. 



Nitrites produced from nitrates. 



Brown to dark brown soluble pigment 

 formed. 



Starch is hydrolyzed. 



Aerobic . 



Optimum temperature 37°C. 



Habitat: Soil. 



14. Streptomyces fimicarius (Duche) 

 comb. 710V. {Actinomyces fitiucanus 

 Duche, Encyclopedie Mycologique, Paris, 

 6, 1934, 346.) From Latin fimus, tlung 

 and caruSy loving. 



Gelatin : Punctiform colonies with 

 whitish aerial mycelium; reddish soluble 

 pigment. Liquefaction. 



Asparagine agar : Cream-colored growth 

 with whitish aerial mycelium; reverse 

 side, cream-colored to slight ochre. 



Czapek's agar : Yellowish masses of 

 growth with yellowish-white aerial my- 

 celium ; reverse side orange-colored ; faint 

 yellowish soluble pigment. 



Peptone agar : Cream-colored growth 

 with white aerial mj'celium; reverse side, 

 yellowish. 



Asparagine solution : Vegetative fila- 

 ments 0.5 to 0.6 micron long; branching 

 aerial mycelium 0.8 to 1.0 micron, forming 

 numerous conidia ; flaky growth produced 

 on bottom; surface growth becomes cov- 

 ered with a white aerial mycelium ; re- 

 verse side, brownish-red. 



Czapek's solution: Cream-colored 



punctiform growth with yellowish aerial 

 mycelium; no soluble pigment. 



Peptone solution : Whitish growth that 

 flakes throughout liquid; yellowish pig- 

 ment. 



Tyrosine medium: White growth with 

 yellowish reverse ; yellowish soluble pig- 

 ment. 



Milk : Colorless growth becoming cov- 

 ered with whitish aerial mycelium; slow 

 peptonization of milk which becomes rose- 

 colored, finally changing to brownish-red. 



Potato : Cream-colored to yellowish 

 growth with whitish aerial mycelium; 

 reddish-brown pigmentation of plug. 



Coagulated serum : Cream-colored 

 growth with whitish aerial mycelium; 

 rapid liquefaction of serum. 



Distinctive characters : Abundant 

 growth upon neutral and acid media; 

 whitish aerial mycelium; marked odor; 

 soluble brownish-red pigment. This spe- 

 cies seems to form the transition type 

 between the Actinomyces albus group and 

 the Actinomyces chromogenus group. 



Habitat : Found abundantly in manure. 



15. Streptomyces flavovirens (Waks- 

 man) comb. nov. (Actinomyces 128, 

 Waksman, Soil Science, 8, 1919, 117; 

 Actinomyces flavovirens Waksman, in 

 Manual, 1st ed., 1923, 352; Actinomyces 

 albcviridis Duche, Encyclopedie ISIyco- 

 logique, Paris, 6, 1934, 317.) From Latin 

 flauus, yellow and virens, becoming green. 



Large masses of minute tufts ; the hy- 

 phae coarse, straight, short, relatively 

 unbranched, beaded; open spirals may be 

 produced in certain substances. Conidia 

 spherical, oval to rod-shaped, 0.75 to 1.0 

 by 1.0 to 1.5 microns. 



Gelatin stab: Yellowish-green surface 

 pellicle, consisting of a mass of small 

 colonies, on the liquefied medium. 



Synthetic agar : Growth spreading deep 

 into the substratum, yellowish with 

 greenish tinge. Aerial mycelium, gray, 

 powdery . 



Starch agar : Greenish-yellow, spread- 

 ing growth, developing deep into the 

 medium. 



