FAMILY STREPTOMYCETACEAE 



941 



Glucose agar; Restricted growth, de- 

 veloping only to a very small extent into 

 the medium, yellow, turning black, edge 

 entire. 



Plain agar: Yellowish growth; the re- 

 verse dark in center with yellowish zone 

 and outer white zone. 



Glucose broth: Thick, sulfur-yellow 

 pellicle or ring. 



Litmus milk : Cream-colored to brown- 

 ish ring; coagulated; peptonized, becom- 

 ing faintly alkaline. 



Potato : Sulfur-yellow, wrinkled 

 growth. 



Only a trace of nitrite is formed from 

 nitrates. 



Greenish-yellow soluble pigment 

 formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Habitat: Soil. 



16. Streptomyces olivochromogenus 

 (Bergey et al.) comb. nov. (Actinonujces 

 chromogenus 205, Waksman, Soil Science, 

 8, 1919, 106; Actinomyces olivochromog- 

 enus Bergey et al.. Manual, 2nd ed., 1925, 

 36S.) From Greek, producing an olive 

 color. 



Filaments with numerous close spirals. 

 Conidia oval or elliptical. 



Gelatin stab : Cream-colored, spreading 

 surface growth. Rapid liquefaction. 



Synthetic agar: White, spreading 

 growth. Aerial mycelium ash gray with 

 brownish tinge. 



Starch agar: Transparent, spreading 

 growth. 



Glucose agar: Abundant, natal brown 

 to almost black growth, entire margin. 



Plain agar: Wrinkled, brown growth, 

 becoming gray -green. 



Glucose broth: Thin, brown growth, 

 flaky sediment. 



Litmus milk : Dark brown ring ; coagu- 

 lated; peptonized, becoming alkaline. 



Potato : Small, wrinkled, black colonies. 



Faint traces of nitrites formed from 

 nitrates. 



Soluble brown pigment formed. 

 Starch is hydrolyzed. 

 Aerobic . 



Optimum temperature 37 °C. 

 Habitat: Soil. 



17. Streptomyces diastatochromo- 

 genes (Krainsky) comb. nov. {Actino- 

 myces diastatochromogenes Krainsky, 

 Cent. f. Bakt., II Abt., 41, 1914, 662.) 

 From Greek, probably intended to mean 

 producing both diastase and color. 



Conidia spherical or oval, about 1.2 

 microns. 



Gelatin colonies : Light gray-colored. 



Gelatin stab: Liquefied. 



Plain agar: Medium-sized colonies, 

 with white to gray aerial mycelium. 



Ca-malate agar : Medium-sized colonies, 

 colorless, with gray aerial mycelium. 



Glucose agar: Same as on Ca-malate 

 agar. 



Starch agar : Same as on Ca-malate agar. 



Glucose broth : Flaky colonies in depth 

 at first, later also over surface. 



Potato : Light gray colonies ; gray aerial 

 nwcelium; medium colored black. 



Soluble brown pigment formed in 

 gelatin. 



Weakly diastatic. 



Xo growth on cellulose. 



Tyrosinase formed. 



Aerobic. 



Optimum temperature 3o°C. 



Habitat: Soil. 



18. Streptomyces flavochromogenes 



(Krainsky) comb. nov. {Actinomyces 

 flavochromogenes Krainsky, Cent. f. 

 Bakt., II Abt., 41, 1914, 662.) From 

 Latin flavus, yellow and Greek, produc- 

 ing color. 



Conidia oval, 1.7 microns. 



Gelatin colonies : Yellowish colonies. 



Gelatin stab : Slight liquefaction. 



Plain agar : Aerial mycelium formed 

 late, at first white, later gray. Gray 

 soluble pigment formed. 



Ca-malate agar : Colonies yellow with 

 white aerial mycelium forming late. 



