FAMILY STREPTOMYCETACEAE 



945 



glucose agar are sinistrorse. Conidia 

 spherical, 1.0 to 1.4 microns in diameter. 



Gelatin stab: Liquefied with small, 

 brown flakes. 



Synthetic agar: Colorless growth, with 

 yellowish tinge, becoming brownish, 

 spreading. Aerial mycelium thin, white, 

 cottony. 



Starch agar: Brownish-gray growth. 



Glucose agar: Restricted, brownish 

 growth, center raised. 



Plain agar : Gra}^ wrinlded growth, be- 

 coming brownish. 



Glucose broth : Sediment consisting of 

 large colonies. 



Litmus milk: Reaction unchanged; co- 

 agulated; peptonized. 



Potato: Gray growth, with black 

 center. 



Nitrites produced from nitrates. 



Dark brown pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Source : From upland and adobe soils in 

 California. 



Habitat: Soil. 



27. Streptomyces rubrireticuli nom. 

 nov. (Actinomyces reliculus -ruber Waks- 

 man, Soil Science, 8, 1919, 146; Actino- 

 myces reticulus Bergey et al., Manual, 

 2nd ed., 1925, 373.) From Latin ruber, 

 red and reticulum, a small net. 



Branching filaments with both primary 

 and secondary whorl formation. Spirals 

 formed on glucose agar. Conidia oval- 

 shaped. 



Gelatin stab : Surface growth yellow- 

 ish-red to dragon-pink. Liquefied. 



Synthetic agar: Abundant, spreading 

 growth, usually pink. Aerial mycelium 

 thin, rose to pink. 



Starch agar : White growth with red 

 tinge. 



Glucose agar: Abundant, spreading, 

 rose-red, entire growth. 



Plain agar : Red growth, with yellowish 

 margin, becoming red. 



Glucose broth : Thin, flaky sediment. 



Litmus milk : Abundant, red pellicle ; 

 coagulated; peptonized. Reaction un- 

 changed. 



Potato : Cream-colored growth, later 

 pink to dark red. 



Nitrites produced from nitrates. 



Soluble dark brown pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



Certain strains of this organism pro- 

 duce an antibiotic. 



Source : Isolated from New Jersey 

 orcliard and California upland soils. 



Optimum temperature 37°C. 



Habitat: Soil. 



28. Streptomyces flavus (Krainsky) 

 cornb. nov. (Actinomyces flavus Krain- 

 sky, Cent. f. Bakt., II Abt., 41, 1914, 662; 

 Waksman and Curtis, Soil Science, /, 

 1916, 118; not Actinomyces flavus San- 

 felice. Cent. f. Bakt., I Abt., Orig., 36, 

 1905, 359.) From Latin ^ai^ws, yellow. 



Coarse filaments with branching hy- 

 phae. Conidia formed by budding and 

 breaking up of hyphae into oval forms. 



Gelatin stab: Small, yellowish masses 

 on surface of liquefied medium. 



Synthetic agar : Circular, yellow or sul- 

 fur-yellow colonies. Aerial mycelium 

 straw-yellow. 



Starch agar: Spreading, cream-colored 

 growth, with pink tinge. 



Glucose agar : Restricted, raised, folded, 

 sulfur-yellow growth, center shading to 

 brown. 



Plain agar: Gray, spreading, folded 

 growth. 



Glucose broth : Small, white colonies in 

 bottom of tube. 



Litmus milk: Coagulated; peptonized, 

 becoming distinctly alkaline. 



Potato: Elevated, much wrinkled, 

 greenish-olive growth. , 



Traces of nitrite formed. 



Soluble brown pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



