946 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Optimum temperature 25°C. 

 Habitat: Soil. 



29. Streptomyces ruber (Krainsky) 

 comb. nov. (Actinomyces ruber Krain- 

 sky, Cent, f . Bakt., II Abt., 41, 1914, 662 ; 

 Waksman, Soil Science, 8, 1919, 149; not 

 Actinomyces ruber Sanfelice, Cent. f. 

 Bakt., I Abt., Orig., 36, 1904, 355; No- 

 cardia krainskii Chalmers and Chris - 

 topherson, Ann. Trop. Med. and Para- 

 sit., 10, 1916, 268.) From Latin ruber, 

 red. 



Straight, branching mycelium, radiat- 

 ing. A few spirals may be formed. 



Gelatin stab : Liquefaction, with yellow 

 flakes. 



Synthetic agar: Abundant, spreading, 

 red growth. Aerial mycelium abundant, 

 cottony, chrome-orange. 



Starch agar : Abundant, spreading, red 

 growth . 



Glucose agar: Restricted, abundant, 

 entire, coral-red growth. 



Plain agar : Restricted, elevated, wrin- 

 kled, olive-green growth. 



Glucose broth : Red ring, with spongy 

 colonies on the surface. 



Litmus milk : Dark ring with red tinge ; 

 coagulated; peptonized, with alkaline 

 reaction . 



Potato: Elevated, wrinkled, greenish 

 growth. 



Nitrites produced from nitrates. 



Soluble brown pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 37 °C. 



Habitat: Soil. 



30. Streptomyces citreus (Krainsky) 

 comb. nov. {Actinoynyces citreus Krain- 

 sky, Cent. f. Bakt., II Abt., 41, 1914, 662; 

 Waksman and Curtis, Soil Science, 1, 

 1916, 99; not Actinomyces citreus Gas- 

 perini,- Cent. f. Bakt., 15, 1894, 684.) 

 FromM. L. citreus, lemon-yellow. 



Filaments with long, narrow open 

 spirals. Conidia spherical to oval, 1.2 

 to 1 .5 by 1 .2 to 1 .8 microns. 



Gelatin stab : Yellowish, restricted sur- 

 face growth. Liquefaction in 35 days. 



Synthetic agar: Abundant, spreading, 

 raised, wrinkled, citron-yellow growth. 

 Aerial mycelium covering surface ; citron- 

 yellow. 



Starch agar : Abundant, yellowish- 

 green growth. 



Glucose agar: Extensive, glossy, olive- 

 yellow, entire growth; center elevated. 



Plain agar : Restricted, cream-colored 

 growth . 



Glucose broth : Thin, wide, yellow ring ; 

 flaky sediment. 



Litmus milk : Cream-colored surface 

 growth; coagulated; peptonized, becom- 

 ing alkaline. 



Potato: Yellowish growth, aerial my- 

 celium white. 



Trace of nitrite production from ni- 

 trate. 



The pigment formed is not soluble. 



Starch hydrolyzed. 



Aerobic. 



Optimum temperature 37°C. 



Habitat: Soil. 



31. Streptomyces fulvissimus (Jensen) 

 comb. nov. {Actinomyces fulvissimus 

 Jensen, Soil Science, 30, 1930, 66.) From 

 ha,tin fulvissimus, very yellow. 



Vegetative mycelium without any spe- 

 cial characteristics; aerial mycelium of 

 short, straight, often trifurcated hyphae, 

 1.0 to 1.2 microns broad; no spiral forma- 

 tion; branches of hyphae break up into 

 conidia, 1.0 to 1.2 by 1.2 to 1.5 microns. 



Gelatin : Vegetative mycelium narrow, 

 smooth, yellowish-brown to red-brown; 

 no aerial mycelium; no pigment; gelatin 

 completely liquefied in 10 to 12 days. 



Nutrient agar : Good growth ; vegetative 

 mycelium raised, finely wrinkled, deep 

 red-brown ; no aerial mycelium ; brownish- 

 yellow pigment. 



Czapek's agar : Good growth (one strain 

 very scant), vegetative mycelium flat, 

 narrow, first light golden, later deep 

 orange to red-brown; aerial mycelium 

 scant, sometimes almost absent, first 



