950 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



38. Streptomyces olivaceus (Waks- 

 man) comb. nor. (Actinom3'ces 206, 

 Waksman, Soil Science, 7, 1919, 117; 

 Actinomrjces olivaceus Waksman, in Man- 

 ual, 1st ed., 1923, 354.) From Latin, 

 olive -colored. 



Small clumps, with straight and 

 branching hyphae. No spirals on most 

 media. Conidia spherical and oval, 0.9 

 to 1.1 by 0.9 to 2.0 microns. 



Gelatin stab : Liquefied with cream- 

 colored, flaky, yellow sediment. 



Synthetic agar: Growth abundant, 

 spreading, developing deep into medium, 

 yellow to olive-ochre, reverse yellow to 

 almost black. Aerial mycelium mouse - 

 gray to light drab. 



Starch agar: Thin, yellowish-green, 

 spreading growth. 



Glucose agar: Growth abundant, re- 

 stricted, entire, center raised. 



Plain agar : White, glistening growth. 



Glucose broth : Sulfur-yellow ring. 



Litmus milk : Faint, pinkish growth ; 

 coagulated; peptonized, becoming alka- 

 line. 



Potato: Growth abundant, much 

 wrinkled, elevated, gray, turning sulfur- 

 yellow on edge. 



Nitrites produced from nitrates. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Habitat: Soil. 



39. Streptomyces lieskei (Duche) 

 coynh. nov. {Aclinoviyces lieskei Duche, 

 Encyclopedic M5^cologique, Paris, 6, 

 1934, 289.) Named for Prof. Lieske of 

 Leipzig. 



Gelatin: Cream-colored growth be- 

 coming covered with white aerial myce- 

 lium; no soluble pigment. Rapid 

 liquefaction. 



Plain agar : Cream-colored growth be- 

 coming covered with white aerial myce- 

 lium; yellowish soluble pigment. 



Synthetic agar : Cream-colored growth 

 with delayed white aerial mycelium grow- 

 ing from the edge toward the center; 



mycelium later yellowish. Reverse of 

 growth yellowish to green. Dirty yellow 

 to yellow-green soluble pigment. 



Synthetic solution : Long branching 

 filaments 0.7 micron in diameter. Yel- 

 lowish-white aerial mycelium does not 

 readily produce spores ; flakes drop to 

 the bottom of the tube. 



Peptone solution : Cream-colored colo- 

 nies on surface with flakes in the liquid 

 dropping to the bottom of the tube. 

 Liquid becomes yellowish in color. 



Tyrosine medium : Rapid growth on 

 surface with whitish-yellow aerial myce- 

 lium; yellowish to orange-yellow soluble 

 pigment. 



Milk: Cream-colored growth; colorless 

 on reverse side; no aerial mycelium. 

 Peptonization without coagulation. 

 After 20 days the whole milk becomes a 

 clear yellowish liquid. 



Coagulated serum : Clear-colored 

 growth. Rapid liquefaction. 



Culture related to Streptomyces albo- 

 jlavus and Streptomyces albidoflavus. 



40. Streptomyces microflavus (Krain- 

 sky) comb. nov. {Actinomyces micro- 

 flavus Krainsky, Cent. f. Bakt.', II Abt., 

 4/, 1914, 662; Micromonospora microflava 

 Duche, Encyclopedic Mycologique, 

 Paris, 6, 1934, 29.) From Greek micnis, 

 small, and Liat'in flavus, yellow. 



Conidia large, spherical to rod-shaped, 

 often in pairs or chains, 2.0 by 2.0 to 5.0 

 microns. 



Gelatin colonies: Small, yellow. 



Gelatin stab: Liquefied. 



Plain agar: Yellow colonies, with rose- 

 yellow aerial mycelium in 3 to 4 weeks. 



Ca-malate agar : Minute yellow colo- 

 nies. No aerial mycelium. 



Glucose agar: A rose-yellow aerial my- 

 celium develops in about 12 days. 



Starch agar : Same as on glucose agar. 



Glucose broth : Small spherical colonies 

 in depth. 



Litmus milk: Peptonized. 



Potato: Yellow growth. No aerial 

 mycelium. 



Nitrites produced from nitrates. 



