952 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Starch is hydrolyzed. 

 Aerobic. 



Optimum temperature 37°C. 

 Source : Isolated several times from 

 adobe and upland soils in California. 

 Habitat : Soil. 



44. Streptomyces gelaticus (Waks- 

 man) comb. nov. (Actinomyces 104, 

 Waksman, Soil Science, 8, 1919, 165; 

 Actinomyces gelaticus Waksman, in 

 Manual, 1st ed., 1923, 356.) From M. L. 

 gelaticus, gelatinous. 



Branching mycelium with open spirals. 



Gelatin stab : Liquefied with cream- 

 colored flaky sediment. 



Synthetic agar: Growth colorless, 

 spreading, chiefly deep into the medium. 

 Aerial mycelium thin, white, turning 

 grayish. 



Starch agar: Thin, spreading, cream- 

 colored growth. 



Glucose agar: Abundant, spreading, 

 white growth. 



Plain agar: Wrinkled, cream-colored 

 growth only on the surface. 



Glucose broth : Thin, cream-colored 

 pellicle; slight flaky sediment. 



Litmus milk : Pinkish ring ; coagulated ; 

 peptonized with distinctly alkaline reac- 

 tion. 



Potato: Growth abundant, much 

 wrinkled, greenish, becoming black with 

 yellowish margin. 



Nitrates show slight reduction to ni- 

 trites. 



Soluble brown pigment formed. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Habitat: Soil. 



45. Streptomyces rutgersensis (Waks- 

 man and Curtis) comb. nov. (Actino- 

 myces rutgersensis Waksman and Curtis, 

 Soil Science, 1, 1916, 123.) Named for 

 Rutgers University, New Brunswick, 

 New Jersey. 



Branching filaments with abundant 

 open and closed spirals ; hyphae fine, long, 

 branching. Conidia spherical and oval, 



1.0 to 1.2 microns, with tendency to 

 bipolar staining. 



Gelatin stab : Cream-colored, spreading 

 surface growth. Liquefied. 



Synthetic agar : Growth thin, colorless, 

 spreading, becoming brownish to almost 

 black. Aerial mj'celium thin, white, 

 becoming dull -gray. 



Starch agar : Gray, spreading growth. 



Glucose agar : Abundant, brown myce- 

 lium, becoming black with cream-colored 

 margin. 



Plain agar: Thin, wrinkled, cream- 

 colored growth. 



Litmus milk: Cream-colored ring; 

 coagulated; slow peptonization, becoming 

 alkaline. 



Potato: Abundant, white-gray, much 

 folded growth. 



Nitrites produced from nitrates. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 37 °C. 



Source : Isolated many times from a 

 variety of soils. 



Habitat: Common in soil. 



46. Streptomyces lipmanii (Waksman 

 and Curtis) comb. nov. {Actinomyces 

 lipmanii Waksman and Curtis, Soil 

 Science, 1, 1916, 123.) Named for Prof. 

 J. G. Lipman, New Jersey Agricultural 

 Experiment Station. 



Straight, branching mycelium and 

 hyphae. Conidia oval, 0.8 to 1.1 by 1.0 

 to 1.5 microns. 



Gelatin stab : Liquefied with cream- 

 colored, flaky sediment. 



Synthetic agar : Growth abundant, 

 raised, colorless, becoming light brown 

 and wrinkled. Aerial mycelium white, 

 turning gray. 



Starch agar: Transparent growth, be- 

 coming dark with age. 



Glucose agar : Light yellow, irregular, 

 spreading growth. 



Plain agar: Yellow, glossy, radiately 

 wrinkled growth. 



Glucose broth: White ring, with abun- 

 dant, colorless flaky sediment. 



