954 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Starch is hydrolyzed. 



Cellulose is decomposed readily by 

 some strains. 



Distinctive character: In this species, 

 the aerial mycelium (which in other 

 actinomycetes is strikingly hydrophobic) 

 on certain media (glucose or glycerol 

 asparagine agar) becomes moistened and 

 exhibits dark, glistening patches. These 

 patches, when touched with a needle, 

 prove to be a moist, smeary mass of 

 spores. This characteristic feature is 

 not equally distinct in all strains. 



Source : Seven strains isolated from 

 soils. 



Habitat : Soil . 



49. Streptomyces fradiae (Waksman 

 and Curtis) comb. nov. {Actinomyces 

 f radii Waksman and Curtis, Soil Science, 

 1, 1916, 125.) From the name of a per- 

 son. 



Straight, branching filaments and hy- 

 phae. No spirals. Conidia rod- or oval- 

 shaped, 0.5 by 0.7 to 1.25 microns. 



Gelatin stab : Cream-colored to brown- 

 ish, dense growth on liquid medium. 



Synthetic agar: Smooth, spreading, 

 colorless growth. Aerial mycelium 

 thick, cottony mass covering surface, 

 sea-shell pink. 



Starch agar : Spreading, colorless 

 growth. 



Glucose agar: Growth restricted, 

 glossy, buff-colored, lichenoid margin. 



Plain agar : Growth yellowish, becoming 

 orange-yellow, restricted. 



Glucose broth: Dense, narrow, orange- 

 colored ring; abundant, flak}?^, colorless 

 sediment. 



Litmus milk : Faint, cream-colored 

 ring; coagulated; peptonized, becoming 

 alkaline. 



Potato: Restricted, orange -colored 

 growth. 



Nitrites not produced from nitrates. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 25°C. 



Source : Isolated once from adobe soil 

 in California. 

 Habitat: Soil. 



50. Streptomyces alboflavus (Waks- 

 man and Curtis) comb. nov. {Actinomyces 

 albofiamts Waksman and Curtis, Soil Sci- 

 ence, 1, 1916, 120.) From Latin albus, 

 white and flavus, yellow. 



Straight, branching mycelium, with 

 very little tendency to form spirals. 

 Very few oval -shaped conidia formed. 



Gelatin stab : Abundant, colorless sur- 

 face growth. Liquefaction occurs in 35 

 days. 



Synthetic agar: Growth glossy, color- 

 less, spreading, becoming yellowish. 

 Aerial mycelium white, powdery, with 

 yellow tinge. 



Starch agar : Thin, yellowish, spread- 

 ing growth. 



Glucose agar: Growth restricted, 

 much-folded, creamy with sulfur-yellow 

 surface . 



Plain agar : Restricted, cream-colored 

 growth. 



Glucose broth : White, cylindrical 

 colonies on surface, later flaky mass in 

 bottom of tube. 



Litmus milk : Pinkish ring. No coagu- 

 lation. Peptonized, becoming alkaline. 



Potato : Moist, cream -colored, wrinkled 

 growth. 



Nitrites produced from nitrates. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 37°C. 



Source : Isolated once from orchard soiL 



Habitat: Soil. 



51. Streptomyces albosporeus (Krain- 

 sky) comb. nov. {Actinomyces albo- 

 sporeus Krainsky, Cent. f. Bakt., II 

 Abt., 41, 1914, 649; Nocardia albosporea 

 Chalmers and Christopherson, Ann. 

 Trop. Med. and Parasit., 10, 1916, 268; 

 Waksman and Curtis, Soil Science, 1, 

 1916, 99.) From Latin albus, white and 

 Greek spora, spore. 



