FAMILY STREPTOMYCETACEAE 



955 



Straight, branching filaments with 

 straight, branching hyphae, and occa- 

 sional spirals. Conidia spherical or oval, 

 0.8 to 1.2 by 1.0 to 1.8 microns. 



Gelatin stab : Growth yellow, changing 

 to red, with hyaline margin. Lique- 

 faction in 35 days. 



Synthetic agar: Growth spreading, 

 colorless with pink center, becoming 

 brownish. Aerial mycelium white at 

 first, later covering the surface. 



Starch agar: Growth thin, spreading, 

 transparent, with red tinge. 



Glucose agar: Growth spreading, red, 

 wrinkled, radiate, entire. 



Plain agar: Minute, cream-colored 

 colonies . 



Glucose broth : Pinkish ring. 



Litmus milk: Scant, pink ring. No 

 coagulation. No peptonization. 



Potato: Growth thin, spreading, wrin- 

 kled, graj'', becoming brown with greenish 

 tinge. 



Nitrites produced from nitrates. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 37 °C. 



Habitat: Soil. 



"52. Streptomyces flocculus (Duche) 

 comb. nov. {Actinomyces flocculus 

 Duche, Encyclopedie Mycologique, 

 Paris, 6, 1934, 300.) From Latin, some- 

 what woolly, referring to the appearance 

 of the aerial mycelium. 



Gelatin: Very limited growth. Slow 

 liquefaction. 



Asparagine glucose agar : Weak growth ; 

 limited cream-colored colonies hardly 

 raised above the surface of the medium ; 

 occasionally abundant growth is produced 

 with white aerial mycelium and colorless 

 on reverse side. 



Czapek's agar: Cream-colored growth, 

 later covered with white aerial mycelium ; 

 no soluble pigment. 



Peptone agar: Cream-colored growth, 

 later covered with white aerial mycelium ; 

 no soluble pigment. 



Asparagine glucose solution : Branch- 

 ing immersed filaments, 0.8 micron in 

 diameter; aerial mycelium 1.0 by 1.2 

 microns with numerous conidia; flakes 

 settle to the bottom of the tube. 



Peptone solution : Pointed colonies ; 

 cream-colored on surface of medium. 



Tyrosine medium: Whitish growth 

 without any pigment. 



Milk: Rose-colored growth; slow pep- 

 tonization. 



Potato : Punctiform growth covered 

 with white aerial mycelium ; faint yellow- 

 ish pigment. 



Coagulated serum : Cream-colored 

 growth; fine white aerial mycelium; 

 slow liquefaction of serum. 



Source : Culture obtained from Mr. 

 iVIalengon, an inspector in Morocco. 



53. Streptomyces melanosporeus 

 (Krainsky) comh. nov. {Actinomyces mel- 

 anosporeus Krainsky, Cent. f. Bakt., II 

 Abt., 41, 1914, 662; Nocardia mclano- 

 sporen Chalmers and Christopherson, 

 Ann. Trop. I\Ied. and Parasit., 10, 1916, 

 268.) From Greek melas, black and 

 spora, spore. 



Conidia almost spherical, 1.2 microns 

 in diameter. 



Gelatin colony: Small, r,eddish colo- 

 nies. 



Gelatin stab: Liquefied. 



Ca-malate agar: Colonies red, with 

 black aerial mycelium. 



Glucose agar : Same as on Ca-malate 

 agar. 



Starch agar : Same as on Ca-malate 

 agar. 



Glucose broth: Flaky, orange-red colo- 

 nies adherent to glass. 



Litmus milk: Peptonized. 



Potato : Red colonies with black aerial 

 mycelium. 



Nitrites produced from nitrates. 



Weakly diastatic. 



Grows well on cellulose. Cellulose is 

 decomposed. 



Aerobic. 



