958 



MANUAL OF DETERMINATIVE BACrERIOLOGY 



acid-fast organisms, can be selectively 

 impregnated with carbol-auromin and 

 when exposed to ultraviolet radiation 

 fluoresces bright yellow. This technic 

 confirms Lutman's conclusion that the 

 hyphae are intercellular and grow within 

 the middle lamellae (Richards, Stain 

 Tech., 18, 1943, 91-94). 



Source : Isolated from potato scab le- 

 sions. 



Habitat : Cause of potato scab ; found 

 in soil. 



60. Streptomyces ipomoea (Person and 

 Martin) comb. nov. {Actinomyces ipo- 

 moea Person and Martin, Phytopath., 

 30, 1940, 313.) From M. L. Ipomoea, a 

 generic name. 



Conidia on glucose-casein agar : Oval to 

 elliptical, 0.9 to 1.3 by 1.3 to 1.8 microns. 



Gelatin : After 25 days at 20°C, scanty 

 growth, no aerial mycelium; no soluble 

 pigment; liquefaction. 



Synthetic agar : Abundant growth, 

 mostly on surface of medium, moderately 

 wrinkled, olive -yellow. 



Nutrient agar : Moderate growth in the 

 form of small, shiny, crinkled colonies 

 both on the surface and imbedded in the 

 medium, silver-colored. 



Starch agar : Growth moderate, smooth, 

 deep in medium, ivory-colored. Aerial 

 mycelium white with patches of bluish- 

 green. No soluble pigment. Complete 

 hydrolysis after 12 days. 



Milk : Growth in form of ring ; hydroly- 

 sis, without visible coagulation. 



Potato : Growth moderate, light brown, 

 shiny, wrinkled. No aerial mycelium. 

 No soluble pigment. 



Nitrites are produced from nitrates. 



Starch is hydrolyzed. 



No growth on cellulose. 



Source : From diseased sweet-potato 

 {Ipomoea sp.) tubers and small rootlets 

 from several localities in Louisiana. 



61. Streptomyces fordii (Erikson) 

 comb. 710V. {Actinomyces fordii Erikson, 

 Med. Res. Counc. Spec. Rept. Ser. 203, 

 1935, 15 and 36.) Presumably named for 



the surgeon who first secured the culture. 



Mycelium: Filaments of medium 

 length, no spirals or markedly wavy 

 branches. Short, straight, sparse aerial 

 mycelium. Small oval conidia on potato 

 agar and starch agar. 



Gelatin : No visible growth, slight 

 softening in 20 days ; half-liquefied after 

 40 days. 



Agar: Small, creamy -golden, ring- 

 shaped colonies, and heaped-up patches, 

 becoming golden-brown in color and 

 convoluted. 



Glycerol agar : Extensive, golden- 

 brown, convoluted, thin layer. 



Serum agar : Golden-brown ring-shaped 

 and coiled smooth colonies; no liquefac- 

 tion. 



Ca-agar: Yellow, scale-like closely ad- 

 herent colonies ; scattered white aerial 

 mycelium. 



Blood agar : Innumerable small yellow- 

 ish ring-shaped colonies; no hemolysis. 



Broth : Few flakes at first ; later abun- 

 dant coherent puff ball growth. 



Synthetic sucrose solution : Moderate 

 sediment of minute round white colonies. 



Synthetic glycerol solution : Light 

 white fluffy colonies, minute and in clus- 

 ters. 



Inspissated serum : Innumerable color- 

 less pinpoint colonies ; scant white aerial 

 mycelium; after 15 days colonies large, 

 hollow on reverse side ; margin depressed ; 

 no liquefaction. 



Dorset's egg medium: Minute, cream- 

 colored, elevated colonies, becoming 

 golden-brown, raised, convoluted. 



Milk: Coagulated; brownish surface 

 ring. 



Litmus milk: No change in reaction. 



Potato plug : Yellowish growth in thin 

 line, terminal portion tending to be piled 

 up, scant white aerial mycelium at top 

 of slant; after 12 days, growth abundant, 

 golden-brown, confluent, partly honey- 

 combed, partly piled up. 



Starch not hydrolyzed. 



Tyrosine agar: Reaction negative. 



Source : Human spleen in a case of 

 acholuric jaundice. 



