FAMILY STEEPTOMYCETACEAE 



959 



62. Streptomyces africanus (Pijper 

 and Pullinger) cotnb. nov. {Nocardia 

 africana Pijper and Pullinger, Jour. 

 Trop. Med. and Hyg., 30, 1927, 153; 

 Actinomyces ajricanus Nannizzi, in Pol- 

 laci, Tratt. Micopat. Umana, 4, 1934, 8.) 

 From Latin Africanus, relating to Africa. 



Description from Erikson, Med. Res. 

 Counc. Spec. Rept. Ser. 203, 1935, IS. 



Unicellular branching mycelium form- 

 ing small dense pink colonies with short 

 straight sparse white aerial mycelium. 



Gelatin : Irregular pink flakes ; no 

 liquefaction. 



Agar: A few flat pink discoid colonies. 



Glucose agar : Minute red discrete 

 round colonies and piled up paler pink 

 mass with thin white aerial mycelium. 



Glycerol agar: After 2 weeks, small 

 heaped-up colorless masses with pink 

 tinge around the colorless colonies, mar- 

 gin depressed; after 3 weeks, abundant, 

 piled up, pale pink growth. 



Ca-agar: After 1 week, small, round, 

 colorless colonies with red centers, mar- 

 gins submerged; after 2 weeks, growth 

 bright cherry-red, confluent, with color- 

 less margin. 



Dorset's egg medium : Small colorless 

 blister colonies, partly confluent; be- 

 coming wrinkled, depressed into medium ; 

 slight liquefaction. 



Serum agar: Irregularly round, raised, 

 wrinkled, colorless colonies; becoming 

 dry, pink and flak}-; later piled up, 

 brownish, friable. 



Inspissated serum : After one week, 

 smooth, round, colorless colonies with 

 submerged margin, in confluent patches 

 pink and pitted into medium; after 2 

 weeks, medium broken up, slight lique- 

 faction; after 3 weeks, liquid dried up, 

 colonies umbilicated, raised, dry and 

 friable. 



Broth: Small pink colonies embedded 

 in coherent flocculent mass. 



Synthetic sucrose solution : Small pink 

 granules in sediment after 1 week; 

 colonies of medium size, coherent, after 

 3 weeks. 



Potato agar : Bright red growth, small 



round colonies with colorless submerged 

 margins, and piled up patches with stiflE 

 sparse white aerial mycelium. 



Litmus milk : Bright red surface 

 growth, liquid unchanged after one 

 month ; liquid opaque reddish-purple 

 after 2 months; hydroh'zed, clear wine- 

 red after 3 months. 



Source : From a case of mycetoma of a 

 foot in South Africa. 



63. Streptomyces gallicus (Erikson) 

 comh. nov. {Actinomyces gallicus Erik- 

 son, Med. Res. Counc. Spec. Rept. Ser. 

 203, 1935, 36.) From Latin gallicus, of 

 the Gauls (French). 



Description from Erikson (loc. cit., 

 p. 24). 



Mycelium shows lateral highly refrac- 

 tive bodies which appear almost identical 

 with the singly situated spores found in 

 Micromonos-pora chalceae. 



Gelatin: Scant irregular pink growth; 

 liquefaction very slow, only slight degree 

 in 20 days. 



Agar : A few transparent minute pink 

 colonies ; growth becomes partly con- 

 fluent. 



Glucose agar : Xo growth. 



Glycerol agar : No growth. 



Czapek's agar: No growth. 



Coon's agar : Minute colorless to pink- 

 ish colonies. 



Ca-agar: Glossy pink pinhead colonies. 



Potato agar : Pale pink, moist, granular 

 growth. 



Serum agar: Pinpoint colonies, pink, 

 shining. 



Blood agar : Abundant growth, minute, 

 discrete, round, pink colonies, some 

 aggregated in confluent narrow bands. 

 No hemolysis. 



Dorset's egg medium: Minute colonies, 

 becoming confluent, tangerine-colored. 



Inspissated serum : Abundant, pink, 

 membranous growth, becoming reddish- 

 brown; later discrete colonies at margin, 

 clear on reverse side. No liquefaction. 



Broth : Pinkish flakes. 



Synthetic sucrose solution : A few fine 

 white flocculi. 



