960 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Synthetic glycerol solution : A few 

 small round white colonies. 



Milk: Coagulated; peptonized; j'el- 

 lowish-pink surface ring. 



Litmus milk: No coagulation or pep- 

 tonization; no change in color. 



Potato plug: Very slow growth, a few 

 minute translucent pink colonies after 

 16 days; after 21 days, considerable in- 

 crease in number of colonies, still small 

 and discrete. After 2 months, colonies 

 1 to 2 mm in diameter, bright coral, 

 tending to be umbilicated and heaped up. 



T3'rosine agar: Reaction negative. 



Source : From blood culture in a case 

 of Banti's disease. 



64. Streptomyces pelletieri (Laveran) 

 comb. nov. {Micrococcus pelletieri Lav- 

 eran, Compt. rend. Soc. Biol., Paris, 

 61, 1906, 340; Oospora pelletieri Thiroux 

 and Pelletier, Bull. Soc. path, exot., 5, 

 1912, 585; Nocardia pelletieri Thiroux, 

 see Pinoy, Bull. Inst. Past., 11, 1913, 

 935; Discomyces pelletieri Brumpt, Precis 

 de Parasitol., Paris, 2nd ed., 1913, 970; 

 Actinomyces pelletieri Brumpt, ibid., 

 4th ed., 1927, 1204.) Named for M. 

 Pelletier who first isolated this species. 



Description from Erikson, Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 21. 



Thiroux and Pelletier (Bull. Soc. path, 

 exot., 5, 1912, 585) considered that their 

 cultures resembled Nocardia madurae, 

 but they grew the organism only 

 on Sabouraud's gelatin, on which it 

 appeared in a constantly red, easily de- 

 tachable form. Nocardia indica was 

 regarded as identical by Pinoy, although 

 in the original description by Laveran the 

 organism was called Micrococcus pelle- 

 tieri, owing to the fact that no mycelium 

 was seen, merely coccoid bodies. No- 

 cardia genesii Froes (Bull. Inst. Past., 

 ^9, 1931, 1158) is described as closely 

 allied, the distinction being founded 

 upon the fact that the red grains were 

 smaller in size and much more numerous, 

 but no cultural details are given. 



Mycelium composed of slender straight 

 and not very long filaments, forming small 



dense pink colonies with a few short 

 straight isolated aerial branches. 



Gelatin : Slight liquefaction ; few pink 

 flakes ; later almost completely liquefied. 



Agar : Minute colorless colonies and 

 piled up pale pink masses. 



Glucose agar: Poor growth, a few 

 minute pink colonies. 



Glycerol agar : Poor growth, a few moist 

 pink colonies. 



Ca-agar : Colorless small colonies ; after 

 1 week, confluent skin, pink, buckled; 

 medium discolored later. 



Coon's agar: Poor growth, cream- 

 colored with pink center, mostly sub- 

 merged. 



Potato agar : Colorless blister colonies ; 

 after 3 weeks, colonies larger, showing 

 concentric zones, submerged margins 

 and occasional zone or tuft of white 

 aerial mycelium, pinkish coloration. 



Dorset's egg medium: Abundant, 

 wrinkled, pink skin with small discrete 

 colonies at margin in six days; later 

 surface rough, mealy; considerable lique- 

 faction in 17 days. 



Serum agar: Moist cream-colored 

 growth tending to be heaped up, discrete 

 colonies at margin; becoming umbili- 

 cated. 



Inspissated serum: Round, moist, 

 colorless colonies. 



Blood agar : At first a few pinhead, 

 cream-colored colonies, no hemolysis; 

 later colonies dense, button-shaped, 

 with narrow fringed margin. 



Broth: Small, minute, pink, clustered 

 colonies. 



Synthetic sucrose solution: Small, pink 

 colonies in sediment ; later minute col- 

 onies adhering to side of tube. 



Milk: Soft curd; half -digested; pep- 

 tonization complete in 20 days. 



Litmus milk : Pink surface growth, 

 semi -solid, no color change; after 20 days, 

 coagulum cleared, liquid purple. 



Potato plug: After one month growth 

 sparse; yellowish-pink, irregularly piled 

 up, portions with scant white aerial 

 mycelium; after 6 months abundant 

 highly piled up small rounded pink 



