FAMILY STREPTOMYCETACEAE 



961 



masses, scant white aerial mycelium 

 persistent. 



Source : From a case of crimson-grained 

 mycetoma in Nigeria (E. C. Smith, 

 Trans. R. Soc. Trop. Med. Hyg., 22, 

 1928, 157). 



Habitat : Human infections .so far as 

 known . 



65. Streptomyces listeri (Erikson) 

 comb. 710V. {Actinomyces listeri Erikson, 

 Med. Res. Council Spec. Rept. Ser. 203, 

 1935, 36.) Named for Dr. Joseph Lister, 

 the father of antiseptic surgery. 



Description from Erikson (loc. cil., 

 p. 23). 



Long slender filaments, many loosely 

 wav}% forming a dense spreading my- 

 celium which rapidly grows into a mem- 

 brane on most media. Aerial mycelium 

 very slow and inconstant in appearance, 

 short and straight, conidia oval. 



Gelatin: Slight liquefaction; round 

 white surface colonies; after 45 days, 

 confluent skin, almost completely lique- 

 fied. 



Agar: Smooth, round, moist, cream- 

 colored, margin depressed, center ele- 

 vated, closely adherent; becoming um- 

 bilicated, with a myceloid margin. 



Glucose agar: Cream -colored, glisten- 

 ing, pinpoint colonies ; later aggregated 

 in convoluted skin. 



Glycerol agar: Abundant, moist, 

 cream-colored growth, colonies elevated, 

 piled up ; powdery white aerial mycelium. 

 After 20 days, skin deeply buckled; 

 colorless with exuded drops. 



Ca-agar: Poor growth, a slight biscuit- 

 colored membrane. 



Potato agar : After one week, extensive 

 growth, colorless submerged colonies, 

 warted surface; dirty pink coloration 

 after 2 weeks ; scant white aerial my- 

 celium after 4 months. 



Dorset's egg medium: No growth. 



Blood agar: Small, round, cream- 

 colored colonies, smooth translucent 

 surface; no hemolysis. 



Serum agar: Small, irregular, moist, 

 cream-colored colonies, tending to be 



heaped up; later somewhat transparent. 



Inspissated serum: Abundant growth, 

 colorless shiny colonies, centrally ele- 

 vated, becoming confluent. 



Broth: Small, round, white colonies in 

 .sediment. 



Glucose broth: Small, white, nodular 

 colonies; later abundant flocculi. 



Synthetic sucrose solution : Delicate 

 white colonies in suspension and in 

 sediment. 



Litmus milk: Coagulation. No change 

 in reaction. 



Potato plug: Abundant, dull, brown- 

 ish, wrinkled skin with white aerial 

 mycelium; large, stellate, fluffy, white 

 colonies in liquid at base. 



Source : From human material. Strain 

 from Lister Collection. 



Habitat : From human infections so far 

 as known. 



66. Streptomyces upcottii (Erikson) 

 comb. nov. (A new pathogenic form of 

 Streptothrix, Gibson, Jour. Bact. and 

 Path., 23, 1920, 357; Actinomyces upcottii 

 Erikson, Med. Res. Council Spec. Rept. 

 Ser. 203, 1935, 36.) Named for Dr. 

 Harold Upcott, the surgeon who first 

 secured the culture. 



Description from Erikson {loc. cit., 

 p. 22). 



Filaments characteristically long, 

 straight, much interwoven and ramified; 

 typical unicellular mycelium, usually 

 forming medium to large heavy carti- 

 laginous colonies. Gibson states that 

 the threads vary in thickness and show 

 septa, but this has not been confirmed. 

 A very slight transient aerial mycelium 

 appeared on one agar slope, but this has 

 not been repeated on any slide micro- 

 culture on any medium. Slightly acid- 

 fast. 



Gelatin : Abundant flocculent growth 

 along streak, round cream-colored 

 colonies on surface. Partly liquefied in 

 14 days ; complete liquefaction in 2 

 months . 



Agar: Smooth, shining, round, cream- 

 colored colonies, margin submerged. 



