964 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Blood agar: Hemolysis. Growth in 

 uniformly striated colorless bands, occa- 

 sional round colonies at margin. 



Dorset's egg medium: Extensive, very 

 wrinkled, membranous growth, surface 

 bright yellow. After 2 months, consider- 

 able liquefaction. 



Serum agar: Wrinkled, glistening, 

 cream-colored, membranous growth. 



Inspissated serum : Colorless smeary 

 growth, reverse becoming transparent', 

 starting to liquefy at base ; completely 

 liquefied and brown in 12 days. 



Broth : Suspended and sedimented 

 colorless flocculi, some small round 

 colonies. 



Synthetic sucrose solution : Abundant 

 white colonies in coherent mass near 

 bottom of tube; large shell -shaped 

 masses . 



Synthetic glycerol solution: At first, a 

 few round white colonies in suspension; 

 later, large branched feathery mass at 

 bottom . 



Milk: Coagulated; later peptonized. 



Litmus milk : Medium deep blue, be- 

 coming hydrolyzed to clear purple. 



Potato plug: Colorless moist membra- 

 nous growth with scant white aerial my- 

 celium at top of plug. 



Starch is hydrolyzed. 



Tyrosine agar: Reaction negative. 



Source : Human spleen in a case of 

 spleenic anemia. 



Habitat : From human infections so 

 far as known. 



70. Streptomyces kimberi (Erikson) 

 comb. nov. {Actinomyces kimberi Erik- 

 son, Med. Res. Council Spec. Rept. Ser. 

 203, 1935, 36.) Presumably named for 

 the surgeon who first secured the culture. 



Description from Erikson (loc. cit., 

 p. 14). 



Mycelium of long straight profusely 

 branching filaments forming circum- 

 scribed colonies on all media with abun- 

 dant production of short straight and 

 branched aerial mycelium; small round 

 conidia. Non-acid-fast. 



Gelatin: Liquefied. Smooth shining 



colonies becoming powdery white with 

 aerial mycelium, floating on liquefied 

 medium. No pigmentation. 



Agar : Smooth round moist cream- 

 colored colonies, 1 mm in diameter; after 

 17 days, white powdery aerial mycelium. 



Glucose agar: Discrete cream-colored 

 colonies becoming confluent, white aerial 

 mycelium. 



Glycerol agar: M^ist cream-colored 

 colonies becoming confluent, white aerial 

 mj'^celium. 



Potato agar : Extensive growth covered 

 by white powdery aerial mycelium; large 

 colorless exuded droplets. 



Wort agar: Heavy brownish lichenoid 

 colony; after 30 days, a white aerial 

 mycelium. 



Ca-agar : Dull cream-colored scaly 

 growth, covered by chalky white aerial 

 mycelium. 



Coon's agar : Extensive growth, white 

 aerial mycelium in annular arrangement. 



Czapek's agar: Small colonies covered 

 with white aerial mycelium. 



Blood agar : Many large colonies, cream- 

 colored, tough, smooth, glistening, with 

 margin depressed; no hemolysis. 



Serum agar : Moist, cream-colored 

 honeycombed skin, scant white aerial 

 mycelium. 



Dorset's egg medium : Closely adherent 

 scale-like colonies, centrally elevated, 

 with white aerial mycelium. 



Lispissated serum : Rapid spreading 

 growth, discrete round colonies at margin, 

 completely covered with white aerial 

 mycelium, colorless transpired 'drops ; 

 slight softening at base. 



Broth : Small round colonies in sedi- 

 ment in 2 days ; supernatant colonies 

 with white aerial mycelium and large 

 hollow flakes in sediment in 15 days; 

 occasional reddish-brown coloration. 



Synthetic sucrose solution : Round 

 white colonies at bottom; later small 

 stellate colonies in suspension and a few 

 supernatant with white aerial mycelium. 



Synthetic glycerol solution : Round 

 white colonies at bottom; later coherent 

 mulberry-like mass composed of fluffy 



