FAMILY STREPTOMYCETACEAE 



967 



in most media, with a marked tendency 

 to produce loose spirals (water and 

 synthetic glycerol agar) with chains of 

 ellipsoidal conidia. Thick aerial clus- 

 ters may also be formed. 



Gelatin : Minute colorless colonies ; 

 liquefaction. 



Agar : Heavy folded colorless lichenoid 

 growth, rounded elevations covered with 

 white aerial mycelium ; later, submerged 

 margin, round confluent growth, aerial 

 mycelium marked in concentric zones. 



Glucose agar : Colorless wrinkled con- 

 fluent growth with smooth entire margin, 

 large discrete colonies like flat rosettes ; 

 after 4 months, scant white aerial my- 

 celium. 



Glycerol agar : Round smooth cream- 

 colored colonies, heavy texture, margin 

 submerged, stiff sparse aerial spikes ; 

 after 3 weeks, colonies large (up to 10 mm 

 in diameter). 



Ca-agar : Spreading colorless growth, 

 pitting medium, submerged undulating 

 margin; verj^ scant white aerial mj'- 

 celium. 



Coon's agar : Opaque white growth ex- 

 tending irregularl}'^ (up to 3 mm) into 

 medium, margin smooth and submerged, 

 center raised, greenish tinge covered 

 with white aerial mycelium; after 3 

 weeks, margin green, central mass 

 covered by gray aerial mycelium. 



Potato agar: Fair growth, partly sub- 

 merged, covered with grayish-white 

 aerial mycelium; medium becomes dis- 

 colored. 



Blood agar : Heavily textured small 

 drab colonies, aerial mycelium microscop- 

 ical ; no hemolysis . 



Dorset's egg medium: Large, round, 

 colorless, scale-like colonies, radially 

 wrinkled ; growth brownish, medium 

 discolored in 2 weeks. 



Serum agar: Smooth colorless discoid 

 colonies; marked umbilication after 2 

 weeks . 



Broth : Large fluffy white hemispherical 

 colonies, loosely coherent. 



Synthetic sucrose solution : A few large 

 round white colonies with smooth partly 

 zonate margins, lightlj^ coherent in sedi- 

 ment ; later smaller colonies in suspen.=!ion 

 attached to side of tube. 



Milk: Coagulation; one-third pep- 

 tonized. 



Carrot plug: Colorless raised colonies 

 with powdery white aerial mycelium; 

 after 1 month, very much piled up, aerial 

 mycelium gra}'; after 2 months, super- 

 abundant growth around back of plug, 

 confluent, greatly buckled, all-over gray 

 aerial mycelium. 



Source : Streptothricosis of liver (Will- 

 more, Trans. Roj^ Soc. Trop. Med. Hyg., 

 17, 1924, 344). 



Habitat : From human infections so far 

 as known. 



*Appendix: The following names have 

 been used for species of Slreptoniyces. 

 Many of them are regarded as new by 

 their authors merely because they were 

 isolated from a new tj^pe of lesion, or 

 from some animal other than man. 

 Others are inadequately" described species 

 from air, soil or water. Relationships 

 to other better described species are 

 usually very obscure. Some of the 

 species listed here may belong in the 

 appendix to the genus Nocardia. 



Actinomyces aerugineus Wollenweber. 

 (Arb. d. Forschungsinst. f. Kartoffelbau, 

 1920, 16.) From deep scab on potato. 



Actinomyces albidofuscns Neukirch. 

 (Actinomyces albido fiiscus Berestnew, 

 Inaug. Diss., Moskow, 1897; see Cent. f. 

 Bakt., I Abt., 24, 1898, 707; Xeukirch, 

 Inaug. Diss., Strassburg, 1902, 3.) 

 From grain. 



Actinomyces albidus Duche. (Ency- 

 clopedic Mycologique, Paris, 6, 1934, 

 266.) 



Actinomyces alhoatrus Waksman and 



* This appendix was originally prepared by Prof. S. A. Waksman and Prof. 

 A. T. Henrici, May, 1943; it has been developed further by Mrs. Eleanore Heist 

 Clise, Geneva, New York, August, 1945. 



