1016 



MANUAL OF DETERMINATIVE BA( rr^KIOLOCJ^ 



cessful by t he use of an agar medium that 

 contained 0.1 per cent peptone, 0.05 

 per cent beef extract, 0.05 per cent glu- 

 cose, and traces of yeast extract and 

 ferric phosphate. Good growth on broth 

 of this composition was also obtained. 

 Apparently the yeast extract supplied 

 necessarj' growth substances. 



Source: Isolated September 19, 1945 

 from a mixed culture with Fseudomonas 

 corallina, by streaking a piece of Dicttjoiti 

 dichotoma on agar containing 0.2 per 

 cent potassium nitrate. 



Habitat: From seaweed. Beaufort, 

 North Carolina. 



Appendix: Stapp and Bortels (Cent. f. 

 Bakt., II Abt., 90, 1934, 28) described 

 four new obligate cellulose-decomposing 

 species : Cytophaga silvestris, Cytophaga 

 (imdan's, Cytophaga flavicula and Cyto- 

 phaga crocea. The differences between 

 them are small and, while it is impossible 

 to make positive identifications on the 

 basis of present knowledge, they seem 

 to be very similar to Cytophaga hutchin- 

 sonii. In the absence of comparative 

 pure culture studies on the obligate 

 cellulose-decomposing members of the 

 genus, the proper delimination of species 

 is not possible. Their inclusion in keys 

 must await additional information. 



