1106 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



susceptible to inoculations of infected 

 blood, tissue and cultures. Infection of 

 the guinea pig is subclinical in its mani- 

 festations, probably due to the small 

 number of parasites in the blood. No 

 definite anemia accompanies infection. 



Source and habitat : Erythrocj^tes of 

 the Peruvian guinea pig iCavia por- 

 cellus). Has also been encountered in 

 Colombia but not in other parts of the 

 world. Observed in latently infected 

 animals only after splenectomy. The 

 natural mode of transmission is unknown, 

 though the flea may be a possible vector. 



5. Haemobartonella bovis (Donatien 

 and Lestoquard) Weinman. (Donatien 

 and Lestoquard, Bull. Soc. Path. Exot., 

 27, 1934, 652; Bartonella sergenti Adler 

 and Ellenbogen., Jour. Comp. Path, and 

 Therap., 47, 193-i, 221; (?) Bartonella 

 bovis Rodriguez, Rev. del Inst. Llorente, 

 13, 1935, 5; abst. in Bull. Inst. Past., 34, 

 1936, 1033; Weinman, Trans. Amer. 

 Philosoph. Soc, N. S., 33, 1944, 308; 

 Haemobartonella sergenti Weinman, loc. 

 oil., 290.) From Latin bos, bovis, ox. 



Resembles Haemobartonella ynuris and 

 //. canis. Occurs as rods, coccobacilli 

 and cocci, singly, in pairs or short chains 

 or groups of 10 or more elements. The 

 rods measure 1.2 to 2 microns in length 

 and are very slender. The coccobacilli 

 occur singly or in pairs measuring 0.3 

 by 0.6 to 0.8 micron and the diameters of 

 the cocci are about 0.3 micron. The 

 parasite may occupy a central or marginal 

 position on the red cell ; the number on a 

 cell varying from 1 to 20. Not more 

 than 20 per cent of the cells are parasi- 

 tized. 



Using the Romanowsky stain, the or- 

 ganisms stain similarly to the chromatin 

 of Piroplasma spp. 



Source and habitat : In the blood of 

 bulls in Algeria and in a non-splenectom- 

 ized calf in Palestine. 



6. Haemobartonella sturmanii Grin- 

 berg. (Grinberg, Ann. Trop. Med., 33, 



1939, 33; Weinman, Trans. Amer. Philo- 

 soph. Soc, N. S., 33, 1944, 313.) 



Similar to Haemobartonella bovis and 

 H. ca7iis in morphologj^ and staining 

 properties. Occurs as rods, cocco-bacil- 

 lary and coccoid forms, varying in length 

 from 0.5 to 1.5 microns. The number of 

 parasites per infected cell varies from 1 

 to 15 and they occur individually, scat- 

 tered irregularly in clumps or sometimes 

 in chains stretching across the cell. At 

 the height of the infection more than 90 

 per cent of the cells are infected. 



Pathogenicity: Causes a temperature 

 rise in buffaloes and slight anemia after 

 direct blood inoculation. Splenectom- 

 ized rabbits, hamsters and splenectom- 

 ized calves inoculated with blood from 

 infected buffaloes remained free of the 

 parasite . 



Source and habitat: In the blood of 

 buffaloes in Palestine. 



7. Haemobartonella peromysciiTyzzer. 

 (Proc Amer. Philos. Soc, 85, 1942, 377.) 

 Named for the genus of deer mice, Pero- 

 myscus. 



Occurs as delicate filamentous forms 

 (which may be branched) on the red 

 blood cells. These filaments may be- 

 come beaded and give rise to a number of 

 coccoids and rods from which ring forms 

 may develop. 



Stains by Giemsa's method, but stain- 

 ing process must be intense in order to 

 demonstrate the organism. 



Pathogenicity : Infection transmissible 

 to splenectomized white rats, white 

 mice and voles, producing a more or less 

 severe illness with anemia. 



Habitat: In the blood of the deer 

 mouse (Peromyscus lencopns novabor- 

 acensis) . 



7a. Haemobartonella peromyscii var. 

 maniculati Tyzzer. (Proc Amer. Philos. 

 Soc, 85, 1942, 381.) Named for the 

 species of mouse from which it was iso- 

 lated . 



Occurs as rods and filamentous 



