1114 MANUAL OF DETERMINATIVE BACTERIOLOGY 



FAMILY III. CHLAMYDOZOACEAE IMOSHKOVSKY . * 



(Uspekhi Souremennoi Biologii (Russian) (Advances in Modern Biology), 19, 



1945, 12.) 



Small, pleomorphic, often coccoid microorganisms usually with characteristic de- 

 velopment cycle. Stain with aniline dyes. Gram -negative. Behave as obligate 

 intracytoplasmic parasites. Have not been cultivated in cell-free media. Criteria 

 adequate for classification lacking for more recentl}' isolated members. The attribu- 

 tion of Genus III, Colcsiuta, either to Rickettsiaceae or to Chlamydozoaceae is still in 

 doubt . 



Key lo the genera of family Chlamydozoaceae. 



I. Cells coccoid and with life cycle. 



A. Non-oultivatable in chicken embryonic tissues. 



Genus I. Chlamydozoon, p. 1114. 



B. Cultivatable in chicken embryonic tissues. 



Genus II. Miyagawanella, p. 1115. 

 II. Cells pleomorphic. 



Genus III. Colesiota, p. 1119. 



Genus I. Chlamydozoon Ilalberstaedter and von Prowazek. 



fArb. a. d. kaiserl. Gesundheitsamte, 26, 1907, 44.) From Greek chlamydos, cloak 



and zoon, animal. 



Coccoid spherical cells with developmental cycle. Gram-negative. Intracyto- 

 plasmic Iiabitat. Non-cultivatable in chicken embryonic tissues. Susceptible to 

 sulfonamide and penicillin action. 



The type species is Chlamydozoon trachomatis Foley and Parrot. 



1. Chlamydozoon trachomatis Foley dyes; blue or reddish-blue with the 

 and Parrot. (Rickettsia trachomae Bus- Giemsa stain and red or blue, depending 

 acca. Arch. Ophthalm., 52, 1935, 567; on the metabolic state, with the Macchi- 

 Foley and Parrot, Arch. Inst. Past. d'Al- avello stain. Matrix of plaques gives a 

 g4ne, 15, 1937, SS9; Rickettsia trachoma- strong reaction for glycogen. Non- 

 ius Foley and Parrot, ic/e/n.) Named for motile, 

 the disease, trachoma. Cultivation : Has never been culti- 



Coccoid bodies: Small microorganisms yot-gj 



200 to 350 millimicrons in diameter form ^ i • i <- tr 



, ,. T • • , , 1- Immunological aspects: Has one or 



the elementary bodies. Initial bodies ^. . ■^.u ^ t 



.,,. . . ,. ^ 1 nwre antigens in common with or closely 



up to 800 millimicrons in diameter and , ,, ^ • ir- 



r X ir> • 1 f J Ml resembling one or more present m iii ?. va- 

 plaques up to 10 microns also tound. All , * ,^ , • , 

 larger forms encapsulated with substance Sawanella spp. Produces, in low con- 

 derived either from the agent or from centrations, antibodies which fix comple- 

 the cytoplasm of the parasitized cells. me^t with antigen from Miyagawanella 

 Elementary body is the basic unit. lymphogranulomatis. 

 Paired forms or clusters occur. Gram- Pathogenicity: Pathogenic for man, 

 negative. Stains poorly with aniline apes and monkeys where it affects only 



* Prepared by Dr. Geoffrey Hake, The Squibb Institute for Medical Research, 

 New Brunswick, New Jersey, September, 1946. 



