APPENDIX TO RICKETTSIALES 



1121 



* Appendix to Order Rickettsiales : The following are described species of intra- 

 cytoplasmic and intranuclear parasites of Protozoa whose relationships to similar 

 parasites of arthropods and vertebrates are not yet clear. All of the protozoon in- 

 tracellular parasites are of larger size than typical members of Rickettsiales and some 

 have been placed in genera (Cladothrix, Micrococcus) where the typical species do 

 not live intracellularly. 



Genus A. Caryococcus Dangeard. 



(Compt. rend. Acad. Sci., Paris, 1S4, 1902, 1365.) 



Genus established for a bacterial parasite of the nucleus of Euglena; organisms 

 rounded. 

 The type species is Caryococcus hypertrophicus Dangeard. 



1. Caryococcus hypertrophicus Dan- 

 geard. (Compt. rend. Acad. Sci., Paris, 

 1S4, 1902, 1365.) Parasitic in the nucleus 

 of a flagellate {Euglena deses). 



Occurs in the nucleus as an agglomera- 

 tion of close-set, rounded corpuscles. 

 The nucleus increases considerably in 

 volimie, the chromatin is reduced to thin 

 layers against the membrane, the interior 

 of the nucleus is divided into irregular 

 compartments by chromatic trabeculae. 



2. Caryococcus cretus Kirby. (Univ. 

 Calif. Publ. Zool., 49, 1944, 240.) Para- 

 sitic in the nucleus of a flagellate {Tri- 

 chonympha corbula) from the intestine 

 of a termite (Procryptotermes sp.), 

 Madagascar. 



Spherules 1 to 1.5 microns or more in 

 diameter, in preparations appearing clear 

 with usually a chromatic, sharply de- 

 fined, crescentic structure peripherally 

 or interiorly situated, sometimes with 

 two such bodies or several chromatic 

 granules ; parasitic in nucleus ; parasit- 

 ized nucleus enlarged only moderately or 

 not at all, chromatin altered but not 

 greatly diminished in amount . 



3. Caryococcus dilatator Kirby. 

 (Univ. Calif. Publ. Zool., 49, 1944, 238.) 

 Parasitic in the nucleus of flagellates 

 {Trichonympha chattoni and other species 

 of Trichonympha) from the intestine of 



termites {Glyptotermes iridipennis) , Aus- 

 tralia, and other species. 



Spherules 0.5 micron or less in di- 

 ameter, internally differentiated with 

 stainable granule or stainable region pe- 

 ripherally situated; parasitic in nucleus 

 and nucleolus; nuc-^eus becomes greatly 

 enlarged and the c hromatin mostly or 

 entirely disappears. 



4. Caryococcus invadens Kirby. 

 (Univ. Calif. Publ. Zool., 49, 1944, 238.) 

 Parasitic in the nucleus of a flagellate 

 {Trichonympha peplophora) from the in- 

 testine of a termite {Neotermes howa), 

 Madagascar. 



Spherules 1 to 1 .5 microns in diameter, 

 sometimes arranged in pairs, often in- 

 ternally differentiated with stainable 

 central or peripheral granules or stained 

 areas ; parasitic in the nucleolus or endo- 

 some and nucleus; parasitized nucleolus 

 becoming greatly enlarged and crossed by 

 trabeculae, eventually consumed; nu- 

 cleus becoming moderately enlarged, but 

 chromatin not disappearing. 



5. Caryococcus nucleophagus Kirby. 



(Univ. Calif. Publ. Zool., 49, 1944, 236.) 

 Parasitic in the nucleus of a flagellate 

 {Trichonympha corbula) from the intes- 

 tine of termites {Procryptotermes sp.), 

 Madagascar, and three species of Kalo- 

 tennes (s. 1.) from Madagascar. 



* Prepared by Prof. Harold Kirby, Jr. 

 fornia, October, 1946. 



University of California, Berkeley, Call- 



