1132 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Serological relationships : Induces for- 

 mation of antibody capable of neutraliz- 

 ing bacteriophages S18, C38, M, and C37, 

 but not bacteriophages S13, C13, D5, 

 D20, D13, C18, D3, S8, C21, C16, or D6, 

 which represent distinct serological 

 groups . 



Immunological relationships : Member 

 of Resistance Group I. 



Other properties : Particle size, 20 to 30 

 millimicrons. Completely inactivated 

 by 1 : 25,000 methylene blue in 2 mm layer 

 20 cm from 100 candle-power light for 30 

 minutes. 



Literature: Burnet, Jour. Path, and 

 Bad., 36, 1933,307-318. 



4. Phagus primarius H. {loc. cit., 143). 

 From Latin primarius, chief or first. 



Common names : Bacteriophage Do, 

 C51,C50, andD48. 



Hosts : Escherichia coli Castellani and 

 Chalmers ; Shigella dysenteriae Castellani 

 and Chalmers. 



Induced disease : Moderately large 

 plaques, 2 to 6 mm in diameter, witli 

 distinct halos. 



Serological relationships : Cross-neu- 

 tralization reactions with bacteriophages 

 C51, C50, and D48, but not with S13, C13, 

 C36, D20, D13, C18, D3, S8, C21, C16, D6. 



Immunological relationships : Member 

 of Resistance Group I. 



Other properties : Particle size, 20 to 

 30 millimicrons. Completely inactivated 

 by 1 : 25 ,000 methylene blue in 2 mm layer 

 20 cm from 100 candle-power light for 30 

 minutes. 



Literature: Burnet, Jour. Path, and 

 Bact., 36, 1933, 307-318. 



5. Phagus secundarius H. {loc. cit., 

 143). From Latin secundarius, inferior 

 or second. 



Common names : Bacteriophage D20 

 and G. 



Hosts : Escherichia coli Castellani and 

 Chalmers ; Shigella dysenteriae Castellani 

 and Chalmers. 



Induced disease : Moderately lai^e 



plaques, 2 to 6 mm in diameter, with dis- 

 tinct halo. 



Serological relationships : No cross- 

 neutralization reactions with bacterio- 

 phages S13, C13, C36, D5, D13, CIS, D3, 

 S8, C21, C16, or D6. 



Immunological relationships : Member 

 of Resistance Group II. 



Other properties : Nearly all inacti- 

 vated by 1 : 25,000 methylene blue in 2 mm 

 layer 20 cm from 100 candle-power light 

 for 30 minutes. Particle size, 20 to 30 

 millimicrons. 



Literature: Burnet, Jour. Path, and 

 Bact., 36, 1933, 307-318. 



6. Phagus dysenteriae H. {loc. cit., 

 144). Fvoin lidtin dysenteria, dysentery. 



Common names : Bacteriophage D13, 

 specific dysentery phage. 



Host : Shigella dysenteriae Castellani 

 and Chalmers. 



Insusceptible species: Escherichia coli 

 Castellani and Chalmers. 



Induced disease : Moderately large 

 plaques, 2 to 6 mm in diameter, with 

 distinct halo. 



Serological relationships : Antiserum 

 to this strain is not known to be effective 

 against any other strain of bacteriophage ; 

 in particular, no cross reactions with bac- 

 teriophages S13, C13, C36, D5, D20, C18, 

 D3,S8, C21,C16,orD6. 



Immunological relationships : Member 

 of Specific Dysentery Resistance Group. 



Other properties: Particle size, 20 to 

 30 millimicrons. Completely inactivated 

 by 1 : 25,000 methylene blue in 2 mm layer 

 20 cm from 100 candle-power light for 30 

 minutes. 



Literature : Burnet, Jour. Path, and 

 Bact., 36, 1933, 307-318. 



7. Phagus medius H. {loc. cit., 144). 

 From Latin medius, moderate, in refer- 

 ence to particle size. 



Common name : Bacteriophage C18, 

 C35, C26, C47, or C34. 



Hosts : Escherichia coli Castellani and 

 Chalmers; Shigella dysenteriae Castellani 

 and Chalmers. 



