1140 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



bacteriophage to remain viable under 

 certain adverse conditions. 



Common name : Streptococcus bacterio- 

 phage C. 



Hosts : Streptococcus 646, 594, 756, 806. 



Geographical distribution : United 

 States (Ohio, Massachusetts, Connecti- 

 cut). 



Induced disease: Small plaques, the 

 largest about 1.0 nun in diameter. 



Serological relationships : Specific neu- 

 tralization, but no cross reactions with 

 streptococcus bacteriophages A, B, 

 and D. 



Thermal inactivation : At 63° to 65° C 

 in 1 hour. 



Other properties : Withstands storage 

 in 1 :200 phenol at about 5° C for at least 

 261 days ; equally resistant to storage in 

 1 : 10,000 sodium ethyl mercurithiosalicy- 

 late (merthiolate), or to storage without 

 preservatives. 



Literature: Evans, U. S. Pub. Health 

 Ser., Public Health Reports, 49, 1934, 

 1386-1401. 



33. Phagus michiganensis spec. nov. 

 From name of state, Michigan, where this 

 bacteriophage was first isolated. 



Common name : Streptococcus bacterio- 

 phage D. 



Host : Streptococcus 693. 



Geographical distribution: United 

 States (Michigan). 



Induced disease: Small plaques, about 

 0.75 mm in diameter, edges clear-cut, 

 centers clean. 



Serological relationships : Specific neu- 

 tralization, but no cross neutralization 

 with streptococcus bacteriophages A, B, 

 and C. 



Thermal inactivation : At 60° to 63° C 

 in 1 hour. 



Other properties : Withstands storage 

 at about 5°C for at least 261 days. 



Literature: Evans, V. S. Pub. Health 

 Ser., Public Health Reports, 49, 1934, 

 1386-1401. 



34. Phagus fragilis, H. {loc. cit., 159). 

 From haiin fragilis, fragile, in reference 



to easy destruction of this bacteriophage 

 bj'^ light and by concentrated urea solu- 

 tions. 



Common names : Staphylococcus bac- 

 teriophage Au2, Au3, Au4, or D, perhaps 

 bacteriophage H of Gratia. 



Hosts : Staphylococcus aureus Rosen- 

 bach and Staphylococcus albus Rosenbach. 



Geographical distribution : United 

 States . 



Induced disease : Small plaques, 0.2 to 

 1.0 mm in diameter, with sharp edges. 



Serological relationships : Cross-neu- 

 tralization reactions with staphylococcus 

 bacteriophages Aul, Au3, Au4, and D, 

 but not with staphylococcus bacterio- 

 phages Au21, Aul2, A, B, C, or bacterio- 

 phage CI 6. 



Thermal inactivation : At about 57° C 

 in 30 minutes. 



Other properties : Particle diameter 50 

 to 75 millimicrons. Readily inactivated 

 photodynamically. Completely inacti- 

 vated by 27 per cent urea solution in 1 

 hour at 37° C. Lysis not inhibited even 

 by 1.5 per cent sodium citrate in agar 

 medium. 



Literature : Burnet and Lush, Jour. 

 Path, and Bad., 40, 1935, 455-469 ; Burnet 

 and McKie, Austral. Jour. Exp. Biol, and 

 Med. Sci., 6, 1929, 21-31 ; Fisk, Jour. Inf. 

 Dis., 71, 1942, 153-160. 



35. Phagus intermedius H. (loc. cit., 

 160). From Latin intermedius, inter- 

 mediate, in reference to position of this 

 bacteriophage between staphylococcus 

 bacteriophages that multiply readily in 

 broth cultures of host organisms and 

 those that do not. 



Common name : Staphylococcus bac- 

 teriophage Au21. 



Host : Staphylococcus aureus Rosen- 

 bach. 



Geographical distribution : Australia. 



Induced disease : Small plaques, 0.1 to 

 0.3 mm in diameter, with sharp edges. 



Serological relationships : Specific neu- 

 tralization reaction but no cross-neutrali- 

 zation reaction with staphylococcus bac- 

 teriophages Au2 or Aul2. 



